Transfection types, methods and strategies: a technical review

ZX Chong, SK Yeap, WY Ho - PeerJ, 2021 - peerj.com
Transfection is a modern and powerful method used to insert foreign nucleic acids into
eukaryotic cells. The ability to modify host cells' genetic content enables the broad …

Transfer and expression of foreign genes in mammalian cells

A Colosimo, KK Goncz, AR Holmes, K Kunzelmann… - …, 2000 - Taylor & Francis
The transfer of foreign genes into eukaryotic cells, in particular mammalian cells, has been
essential to our understanding of the functional significance of genes and regulatory …

[HTML][HTML] Conceptual and technical aspects of transfection and gene delivery

L Kaestner, A Scholz, P Lipp - Bioorganic & medicinal chemistry letters, 2015 - Elsevier
Genetically modified animals are state of the art in biomedical research as gene therapy is a
promising perspective in the attempt to cure hereditary diseases. Both approaches have in …

A review of the tortuous path of nonviral gene delivery and recent progress

D Sharma, S Arora, J Singh, B Layek - International journal of biological …, 2021 - Elsevier
Gene therapy encompasses the transfer of exogenous genetic materials into the patient's
target cells to treat or prevent diseases. Nevertheless, the transfer of genetic material into …

[HTML][HTML] Viral and nonviral delivery systems for gene delivery

N Nayerossadat, T Maedeh, PA Ali - Advanced biomedical …, 2012 - journals.lww.com
Gene therapy is the process of introducing foreign genomic materials into host cells to elicit
a therapeutic benefit. Although initially the main focus of gene therapy was on special …

Nonviral vectors in the new millennium: delivery barriers in gene transfer

M Nishikawa, L Huang - Human gene therapy, 2001 - liebertpub.com
Development of an efficient method for introducing a therapeutic gene into target cells in
vivo is the key issue in treating genetic and acquired diseases by gene therapy. To this end …

[HTML][HTML] Gene therapy: advances, challenges and perspectives

GAR Gonçalves, RMA Paiva - Einstein (Sao Paulo), 2017 - SciELO Brasil
The ability to make site-specific modifications to the human genome has been an objective
in medicine since the recognition of the gene as the basic unit of heredity. Thus, gene …

[图书][B] Gene transfer and expression: a laboratory manual

M Kriegler - 1990 - books.google.com
A practical manual of protocols for achieving expression of foreign genes in mammalian
cells. It includes some very new techniques such as PCR-based expression. The author …

Appraisal for the potential of viral and nonviral vectors in gene therapy: A review

MH Butt, M Zaman, A Ahmad, R Khan, TH Mallhi… - Genes, 2022 - mdpi.com
Over the past few decades, gene therapy has gained immense importance in medical
research as a promising treatment strategy for diseases such as cancer, AIDS, Alzheimer's …

[HTML][HTML] Delivery systems for gene therapy

S Mali - Indian journal of human genetics, 2013 - ncbi.nlm.nih.gov
The structure of DNA was unraveled by Watson and Crick in 1953, and two decades later
Arber, Nathans and Smith discovered DNA restriction enzymes, which led to the rapid …