Long-term storage does not affect the expression profiles of mRNA and long non-coding RNA in vitrified-warmed human embryos

J Li, L Zhu, J Huang, W Liu, W Han, G Huang - Frontiers in Genetics, 2022 - frontiersin.org
Although vitrification has been widely applied in assisted reproductive technology, it is
unknown whether storage time has any impact on the mRNA and lncRNA expression …

Effects of vitrification and cryostorage duration on single-cell RNA-Seq profiling of vitrified-thawed human metaphase II oocytes

Y Huo, P Yuan, Q Qin, Z Yan, L Yan, P Liu, R Li… - Frontiers of …, 2021 - Springer
Oocyte cryopreservation is widely used for clinical and social reasons. Previous studies
have demonstrated that conventional slow-freezing cryopreservation procedures, but not …

[HTML][HTML] Transcriptomic Analysis of Vitrified–Warmed vs. Fresh Mouse Blastocysts: Cryo-Induced Physiological Mechanisms and Implantation Impact

CY Lee, HN Tsai, EH Cheng, TH Lee, PY Lin… - International Journal of …, 2024 - mdpi.com
Blastocyst vitrification has significantly improved embryo transfer methods, leading to higher
implantation success rates and better pregnancy outcomes in subsequent frozen embryo …

Effect of vitrification temperature and cryoprotectant concentrations on the mRNA transcriptome of bovine mature oocytes after vitrifying at immature stage

F Zhang, ZY Zhang, MD Cai, XX Li, YH Li, Y Lei, XL Yu - Theriogenology, 2020 - Elsevier
The present study aimed to investigate the effect of vitrification temperature (VT) and
cryoprotective agent concentrations (CPAs) on the mRNA transcriptome of bovine mature …

Effect of vitrification on global gene expression dynamics of bovine elongating embryos

E Gutierrez-Castillo, H Ming, B Foster… - Reproduction …, 2021 - CSIRO Publishing
Embryo vitrification involves exposure to high concentrations of cryoprotectants and osmotic
stress during cooling and warming in the cryopreservation process. Many of these factors …

Effect of vitrification on the microRNA transcriptome in mouse blastocysts

X Zhao, H Hao, W Du, H Zhu - PLoS One, 2015 - journals.plos.org
Vitrification is commonly used in the cryopreservation of mammalian blastocysts to
overcome the temporal and spatial limitations of embryo transfer. Previous studies have …

The impact of zygote vitrification timing on pregnancy rate in frozen-thawed IVF/ICSI cycles

S Makieva, C Stähli, M Xie, AV Gil, MK Sachs… - Frontiers in Cell and …, 2023 - frontiersin.org
Introduction: Cryopreservation of bipronuclear (2PN) stage zygotes is an integral part of IVF
laboratory practice in countries with strict embryo culture legislation. Vitrification of zygotes is …

Long‐term storage does not affect the DNA methylation profiles of vitrified‐warmed human embryos

L Zhu, L Sun, W Liu, W Han… - Molecular Reproduction …, 2024 - Wiley Online Library
With the widespread application of embryo cryopreservation in assisted reproductive
techniques, it is necessary to assess the safety of long‐term cryopreservation of human …

[HTML][HTML] Molecular profiling of human oocytes after vitrification strongly suggests that they are biologically comparable with freshly isolated gametes

C Di Pietro, M Vento, MR Guglielmino, P Borzì… - Fertility and sterility, 2010 - Elsevier
To assess the effects of vitrification on the biomolecular profile of oocytes, we analyzed
through real-time reverse transcriptase–polymerase chain reaction eight genes encoding …

Storage duration of vitrified embryos does not affect pregnancy and neonatal outcomes after frozen-thawed embryo transfer

X Li, P Guo, C Blockeel, X Li, L Deng, J Yang… - Frontiers in …, 2023 - frontiersin.org
Background With the refinement of cryopreservation technology, the number of frozen-
warmed embryo transfer (FET) cycles and cryopreserved embryos has increased rapidly …