Transmembrane (TM) domains as simple as a single span can perform complex biological
functions using entirely lipid-embedded chemical features. Computational design has …

General methods for spatiotemporal control of specific endogenous proteins would be
broadly useful for probing protein function in living cells. Synthetic protein binders that bind …

Thrombopoietin (THPO or TPO) is an essential cytokine for hematopoietic stem cell (HSC)
maintenance and megakaryocyte differentiation. Here, we report the 3.4 Å resolution …

Discrete protein assemblies ranging from hundreds of kilodaltons to hundreds of
megadaltons in size are a ubiquitous feature of biological systems and perform highly …

The de novo design of peptides that bind to target proteins could enable binding to specific
epitopes, inhibition of natural interactions, and targeted degradation of proteins. Despite …

Wnt morphogens are critical for embryonic development and tissue regeneration. Canonical
Wnts form ternary receptor complexes composed of tissue-specific Frizzled receptors …

This thesis spans the work completed on two parallel projects, to (i) study the structure and
mechanism of the Type IIS restriction endonuclease PaqCI, and (ii) participate in the …

The development and homeostasis of multicellular organisms requires that their comprising
cells appropriately process multitudes of information within the cellular microenvironment …

Biologics, which encompass peptides, proteins, nucleotides, and cell-based products are
widely used in both therapeutics and diagnostic applications. Compared to small molecule …

C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, eg chemical
and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non …