Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

[PDF][PDF] Single-cell technologies: From research to application

L Wen, G Li, T Huang, W Geng, H Pei, J Yang, M Zhu… - The Innovation, 2022 - cell.com
In recent years, more and more single-cell technologies have been developed. A vast
amount of single-cell omics data has been generated by large projects, such as the Human …

[HTML][HTML] Coronavirus disease-19 and fertility: viral host entry protein expression in male and female reproductive tissues

KE Stanley, E Thomas, M Leaver, D Wells - Fertility and sterility, 2020 - Elsevier
Objective To identify cell types in the male and female reproductive systems at risk for SARS-
CoV-2 infection because of the expression of host genes and proteins used by the virus for …

Single-cell analysis of human ovarian cortex identifies distinct cell populations but no oogonial stem cells

M Wagner, M Yoshihara, I Douagi… - Nature …, 2020 - nature.com
The human ovary orchestrates sex hormone production and undergoes monthly structural
changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in …

The scRNA-seq expression profiling of the receptor ACE2 and the cellular protease TMPRSS2 reveals human organs susceptible to SARS-CoV-2 infection

J Qi, Y Zhou, J Hua, L Zhang, J Bian, B Liu… - International Journal of …, 2021 - mdpi.com
COVID-19 patients always develop multiple organ dysfunction syndromes other than lungs,
suggesting the novel virus SARS-CoV-2 also invades other organs. Therefore, studying the …

[PDF][PDF] Decoding dynamic epigenetic landscapes in human oocytes using single-cell multi-omics sequencing

R Yan, C Gu, D You, Z Huang, J Qian, Q Yang… - Cell Stem Cell, 2021 - cell.com
Developing female human germ cells undergo genome-wide epigenetic reprogramming, but
de novo DNA methylation dynamics and their interplay with chromatin states and …

Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice

JJ Wang, W Ge, QY Zhai, JC Liu, XW Sun, WX Liu… - PLoS …, 2020 - journals.plos.org
Primordial follicle assembly in the mouse occurs during perinatal ages and largely
determines the ovarian reserve that will be available to support the reproductive life span …

Functionally heterogeneous human satellite cells identified by single cell RNA sequencing

E Barruet, SM Garcia, K Striedinger, J Wu, S Lee… - Elife, 2020 - elifesciences.org
Although heterogeneity is recognized within the murine satellite cell pool, a comprehensive
understanding of distinct subpopulations and their functional relevance in human satellite …

Human granulosa cells—stemness properties, molecular cross-talk and follicular angiogenesis

C Dompe, M Kulus, K Stefańska, W Kranc, B Chermuła… - Cells, 2021 - mdpi.com
The ovarian follicle is the basic functional unit of the ovary, comprising theca cells and
granulosa cells (GCs). Two different types of GCs, mural GCs and cumulus cells (CCs) …

Endothelial cell plasticity at the single-cell level

A Pasut, LM Becker, A Cuypers, P Carmeliet - Angiogenesis, 2021 - Springer
The vascular endothelium is characterized by a remarkable level of plasticity, which is the
driving force not only of physiological repair/remodeling of adult tissues but also of …