Recent progress in CRISPR-based microfluidic assays and applications

P Li, H Xiong, B Yang, X Jiang, J Kong… - TrAC Trends in Analytical …, 2022 - Elsevier
In the past few years, CRISPR-based (Clustered regularly interspaced short palindromic
repeats-based) detection techniques have arisen in the field of bioanalysis, molecular …

[HTML][HTML] INO80 and SWR1 complexes: the non-identical twins of chromatin remodelling

O Willhoft, DB Wigley - Current Opinion in Structural Biology, 2020 - Elsevier
The INO80 family of chromatin remodellers are multisubunit complexes that perform a
variety of tasks on nucleosomes. Family members are built around a heterohexamer of RuvB …

Structural mechanism of extranucleosomal DNA readout by the INO80 complex

F Kunert, FJ Metzner, J Jung, M Höpfler, S Woike… - Science …, 2022 - science.org
The nucleosomal landscape of chromatin depends on the concerted action of chromatin
remodelers. The INO80 remodeler specifically places nucleosomes at the boundary of gene …

The nuclear actin-containing Arp8 module is a linker DNA sensor driving INO80 chromatin remodeling

KR Knoll, S Eustermann, V Niebauer… - Nature structural & …, 2018 - nature.com
Nuclear actin (N-actin) and actin-related proteins (Arps) are critical components of several
chromatin modulating complexes, including the chromatin remodeler INO80, but their …

The Arp8 and Arp4 module acts as a DNA sensor controlling INO80 chromatin remodeling

S Brahma, M Ngubo, S Paul, M Udugama… - Nature …, 2018 - nature.com
Nuclear actin and actin-related proteins (Arps) are key components of chromatin remodeling
and modifying complexes. Although Arps are essential for the functions of chromatin …

Megadalton chromatin remodelers: common principles for versatile functions

A Jungblut, KP Hopfner, S Eustermann - Current Opinion in Structural …, 2020 - Elsevier
Highlights•cryo-EM structures of all four families of ATP-dependent chromatin remodelers.•A
common architecture of multi-subunit remodelers.•A unified motor, rotor, stator and grip …

Structural insights into assembly and function of the RSC chromatin remodeling complex

RW Baker, JM Reimer, PJ Carman, B Turegun… - Nature Structural & …, 2021 - nature.com
SWI/SNF chromatin remodelers modify the position and spacing of nucleosomes and, in
humans, are linked to cancer. To provide insights into the assembly and regulation of this …

Structure analysis of proteins and peptides by difference circular dichroism spectroscopy

T Arakawa, M Tokunaga, Y Kita, T Niikura, RW Baker… - The Protein …, 2021 - Springer
Difference circular dichroism (CD) spectroscopy was used here to characterize changes in
structure of flexible peptides upon altering their environments. Environmental changes were …

[HTML][HTML] Swi/snf chromatin remodeling regulates transcriptional interference and gene repression

K Morse, S Swerdlow, E Ünal - bioRxiv, 2023 - ncbi.nlm.nih.gov
Alternative transcription start sites can affect transcript isoform diversity and translation
levels. In a recently described form of gene regulation, coordinated transcriptional and …

Emerging roles of SWI/SNF remodelers in fungal pathogens

VK Balachandra, SK Ghosh - Current Genetics, 2022 - Springer
Fungal pathogens constantly sense and respond to the environment they inhabit, and this
interaction is vital for their survival inside hosts and exhibiting pathogenic traits. Since such …