Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

Eukaryotic transcription occurs on chromatin, where RNA polymerase II encounters
nucleosomes during elongation. These nucleosomes must unravel for the DNA to enter the …

In the eukaryotic nucleus, DNA is packaged in the form of nucleosomes, each of which
comprises about 147 base pairs of DNA wrapped around a histone protein octamer. The …

Unlike other chromatin remodelers, INO80 preferentially mobilizes hexasomes, which can
form during transcription. Why INO80 prefers hexasomes over nucleosomes remains …

Whole-genome sequencing data mining efforts have revealed numerous histone mutations
in a wide range of cancer types. These occur in all four core histones in both the tail and …

Nucleosomes are disrupted during transcription and other active processes, but the
structural intermediates during nucleosome disruption in vivo are unknown. To identify …

Loss of H2A–H2B histone dimers is a hallmark of actively transcribed genes, but how the
cellular machinery functions in the context of noncanonical nucleosomal particles remains …

The critical role of the INO80 chromatin remodeling complex in transcription is commonly
attributed to its nucleosome sliding activity. Here, we have found that INO80 prefers to …

Nucleosome DNA unwrapping and its disassembly into hexasomes and tetrasomes is
necessary for genomic access and plays an important role in transcription regulation …

Recent studies have implicated the nucleosome acidic patch in the activity of ATP-
dependent chromatin remodeling machines. We used a photocrosslinking-based …