3D structure of the transient intermediate of the enzyme–substrate complex of sortase A reveals how calcium binding and substrate recognition cooperate in substrate …

JL Chen, X Wang, F Yang, B Li, G Otting, XC Su - ACS Catalysis, 2023 - ACS Publications
We report the solution structure of an authentic sortase A thioester intermediate, including
the bound substrate. The structure was determined by a combination of site-specific tagging …

Ligand-capped Cobalt (II) multiplies the value of the double-histidine motif for PCS NMR studies

W Zhu, DT Yang, AM Gronenborn - Journal of the American …, 2023 - ACS Publications
In structural studies by NMR, pseudocontact shifts (PCSs) provide both angular and distance
information. For proteins, incorporation of a di-histidine (diHis) motif, coordinated to Co2+ …

A lanthanide tag for a complementary set of pseudocontact shifts

L Topping, AP Welegedara, M Judd… - Chemical …, 2024 - pubs.rsc.org
Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful
restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide …

[HTML][HTML] Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2, 6-dicyanopyridine

SM Tharayil, MC Mahawaththa, A Feintuch… - Magnetic …, 2022 - ncbi.nlm.nih.gov
The paramagnetism of a lanthanoid tag site-specifically installed on a protein provides a rich
source of structural information accessible by nuclear magnetic resonance (NMR) and …

Distinct stereospecific effect of chiral tether between a tag and protein on the rigidity of paramagnetic tag

JL Chen, B Li, B Ma, XC Su - Journal of Biomolecular NMR, 2022 - Springer
Flexibility between the paramagnetic tag and its protein conjugates is a common yet
unresolved issue in the applications of paramagnetic NMR spectroscopy in biological …

Site-selective generation of lanthanoid binding sites on proteins using 4-fluoro-2, 6-dicyanopyridine

S Mekkattu Tharayil, MC Mahawaththa… - Magnetic …, 2022 - mr.copernicus.org
The paramagnetism of a lanthanoid tag site-specifically installed on a protein provides a rich
source of structural information accessible by nuclear magnetic resonance (NMR) and …