Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Meta-analysis of leukocyte diversity in atherosclerotic mouse aortas
The diverse leukocyte infiltrate in atherosclerotic mouse aortas was recently analyzed in 9
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
single-cell RNA sequencing and 2 mass cytometry studies. In a comprehensive meta …
High-speed fluorescence image–enabled cell sorting
Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …
remains a technical challenge. Here, we address this by establishing high-speed image …
Marked T cell activation, senescence, exhaustion and skewing towards TH17 in patients with COVID-19 pneumonia
S De Biasi, M Meschiari, L Gibellini, C Bellinazzi… - Nature …, 2020 - nature.com
The immune system of patients infected by SARS-CoV-2 is severely impaired. Detailed
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
investigation of T cells and cytokine production in patients affected by COVID-19 pneumonia …
Impaired immune cell cytotoxicity in severe COVID-19 is IL-6 dependent
BACKGROUND Coronavirus disease 19 (COVID-19) is an emerging infectious disease
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
caused by SARS-CoV-2. Antiviral immune response is crucial to achieve pathogen …
[PDF][PDF] Precise reconstruction of the TME using bulk RNA-seq and a machine learning algorithm trained on artificial transcriptomes
A Zaitsev, M Chelushkin, D Dyikanov, I Cheremushkin… - Cancer Cell, 2022 - cell.com
Cellular deconvolution algorithms virtually reconstruct tissue composition by analyzing the
gene expression of complex tissues. We present the decision tree machine learning …
gene expression of complex tissues. We present the decision tree machine learning …
[HTML][HTML] SARS-CoV-2–associated ssRNAs activate inflammation and immunity via TLR7/8
V Salvi, HO Nguyen, F Sozio, T Schioppa, C Gaudenzi… - JCI insight, 2021 - ncbi.nlm.nih.gov
The inflammatory and IFN pathways of innate immunity play a key role in the resistance and
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …
pathogenesis of coronavirus disease 2019 (COVID-19). Innate sensors and SARS-CoV-2 …
Altered bioenergetics and mitochondrial dysfunction of monocytes in patients with COVID‐19 pneumonia
L Gibellini, S De Biasi, A Paolini, R Borella… - EMBO molecular …, 2020 - embopress.org
In patients infected by SARS‐CoV‐2 who experience an exaggerated inflammation leading
to pneumonia, monocytes likely play a major role but have received poor attention. Thus, we …
to pneumonia, monocytes likely play a major role but have received poor attention. Thus, we …
A compendium of single extracellular vesicle flow cytometry
JA Welsh, GJA Arkesteijn, M Bremer… - Journal of …, 2023 - Wiley Online Library
Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …
extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells …
Extracellular vesicles as signaling mediators and disease biomarkers across biological barriers
Extracellular vesicles act as shuttle vectors or signal transducers that can deliver specific
biological information and have progressively emerged as key regulators of organized …
biological information and have progressively emerged as key regulators of organized …