Molecular and genetic analyses of Drosophila Prat, which encodes the first enzyme of de novo purine biosynthesis.

DV Clark - Genetics, 1994 - academic.oup.com
Abstract The Drosophila Prat gene encodes phosphoribosylamidotransferase (PRAT), the
enzyme that performs the first committed step of the de novo purine nucleotide biosynthesis …

Synthesis of thiamine in Salmonella typhimurium independent of the purF function

DM Downs, JR Roth - Journal of bacteriology, 1991 - Am Soc Microbiol
In Salmonella typhimurium, the first five steps in purine biosynthesis also serve as the first
steps in the biosynthesis of the pyrimidine moiety of thiamine (vitamin B1). Strains with null …

Identification of sites for feedback regulation of glutamine 5-phosphoribosylpyrophosphate amidotransferase by nucleotides and relationship to residues important for …

G Zhou, H Charbonneau, RF Colman… - Journal of Biological …, 1993 - Elsevier
Glutamine phosphoribosylpyrophosphate amidotransferase, the key regulatory enzyme for
de novo purine nucleotide synthesis, is subject to feedback regulation by adenine and …

Control of de novo purine biosynthesis genes in ureide‐producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization …

JH Kim, AJ Delauney, DPS Verma - The Plant Journal, 1995 - Wiley Online Library
Soybean (Glycine max) and mothbean (Vigna aconitifolia) cDNA clones encoding glutamine
phosphoribosylpyrophosphate amidotransferase (PRAT), the first enzyme of the de novo …

5-Phosphoribosylpyrophosphate amidotransferase from soybean root nodules: kinetic and regulatory properties

PHS Reynolds, DG Blevins, DD Randall - Archives of biochemistry and …, 1984 - Elsevier
Most of the nitrogen transported from the nodules of nitrogen-fixing soybean plants is in the
form of the ureides allantoin and allantoic acid. Recent work has shown that ureides are …

Glutamine-and N-acetyl-L-glutamate-dependent carbamoyl phosphate synthetase from Micropterus salmoides. Purification, properties, and inhibition by glutamine …

CA Casey, PM Anderson - Journal of Biological Chemistry, 1983 - ASBMB
The glutamine-and N-acetyl-L-glutamate-dependent carbamoyl phosphate synthetase III
present in liver of largemouth bass (Micropterus salmoides) has been highly purified. The …

Regulation of Escherichia coli purF. Mutations that define the promoter, operator, and purine repressor gene.

RJ Rolfes, H Zalkin - Journal of Biological Chemistry, 1988 - Elsevier
Mutations were constructed in vitro which identify the-35 promoter element and the operator
site of the Escherichia coli purF operon as well as confirm the-10 promoter sequence. The …

Tryptophan fluorescence monitors multiple conformational changes required for glutamine phosphoribosylpyrophosphate amidotransferase interdomain signaling and …

S Chen, JW Burgner, JM Krahn, JL Smith, H Zalkin - Biochemistry, 1999 - ACS Publications
Single tryptophan residues were incorporated into each of three peptide segments that play
key roles in the structural transition of ligand-free, inactive glutamine …

Structural features of Cryptococcus neoformans bifunctional GAR/AIR synthetase may present novel antifungal drug targets

SMH Chua, MSI Wizrah, Z Luo, BYJ Lim… - Journal of Biological …, 2021 - ASBMB
Cryptococcus neoformans is a fungus that causes life-threatening systemic mycoses. During
infection of the human host, this pathogen experiences a major change in the availability of …

Purification and properties of glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis

JY Wong, DA Bernlohr, CL Turnbough, RL Switzer - Biochemistry, 1981 - ACS Publications
Joseph Y. Wong, David A. Bernlohr, Charles L. Turnbough,* and Robert L. Switzer* abstract:
A procedure for the rapid and efficientpurification of glutamine …