Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type …

BG Mei, H Zalkin - Journal of bacteriology, 1990 - Am Soc Microbiol
A series of deletions was constructed in cloned Escherichia coli purF encoding glutamine
phosphoribosylpyrophosphate amidotransferase. These deletions extended into the NH2 …

Kinetic, physical, and regulatory properties of amidophosphoribosyltransferase

EW Holmes - Advances in Enzyme Regulation, 1981 - Elsevier
Phosphoribosylamine (PRA) is the first unique intermediate in the de novo pathway of
purine ribonucleotide synthesis. Consequently, regulation of the enzyme which catalyzes …

Temperature-dependent function of the glutamine phosphoribosylpyrophosphate amidotransferase ammonia channel and coupling with glycinamide ribonucleotide …

AK Bera, S Chen, JL Smith, H Zalkin - Journal of Bacteriology, 2000 - Am Soc Microbiol
Genes encoding glutamine phosphoribosylpyrophosphate amidotransferase (GPAT) and
glycinamide ribonucleotide synthetase (GARS) from Aquifex aeolicus were expressed in …

[HTML][HTML] Proteome analysis and heterologous cargo delivery of Vibrio natriegens outer membrane vesicles

CUI Yang, D Tao - 微生物学通报, 2021 - wswxtb.ijournals.cn
[Background] The fast-growing Gram-negative bacterium Vibrio natriegens is a burgeoning
tool in biotechnology. Previous research has mainly focused on developing tools for in vitro …

purF-lac fusion and direction of purF transcription in Escherichia coli

JM Smith, JS Gots - Journal of Bacteriology, 1980 - Am Soc Microbiol
The purF locus codes for the first enzyme, glutamine phosphoribosylpyrophosphate
amidotransferase, of the purine biosynthetic pathway. A strain of Escherichia coli K-12 was …

Mutational analysis of the glutamine phosphoribosylpyrophosphate amidotransferase pro-peptide.

JL Souciet, MA Hermodson, H Zalkin - Journal of Biological Chemistry, 1988 - ASBMB
Bacillus subtilis glutamine phosphoribosylpyrophosphate amidotransferase is synthesized
as a pro-enzyme having an 11-amino acid leader. Maturation requires insertion of a [4Fe …

Structure, function, and regulation of amidophosphoribosyltransferase from prokaryotes

H Zalkin - Advances in Enzyme Regulation, 1983 - Elsevier
Recent studies on the structure, function and regulation of amidophosphoribosyltransferase
from E. coli and B. subtilis are reviewed and these properties compared with those of the …

A mathematical model for the adenylosuccinate synthetase reaction involved in purine biosynthesis

EA Oshchepkova-Nedosekina… - Theoretical Biology and …, 2007 - Springer
Background Development of the mathematical models that adequately describe biochemical
reactions and molecular-genetic mechanisms is one of the most important tasks in modern …

A stable carbocyclic analog of 5-phosphoribosyl-1-pyrophosphate to probe the mechanism of catalysis and regulation of glutamine phosphoribosylpyrophosphate …

JH Kim, D Wolle, K Haridas, RJ Parry, JL Smith… - Journal of Biological …, 1995 - ASBMB
Glutamine phosphoribosylpyrophosphate (PRPP) amidotransferase catalysis and regulation
were studied using a new stable carbocyclic analog of PRPP, 1-αpyrophosphoryl-2-α, 3-α …

Crystal Structure of the Chloroplastic Glutamine Phosphoribosylpyrophosphate Amidotransferase GPRAT2 From Arabidopsis thaliana

X Cao, B Du, F Han, Y Zhou, J Ren, W Wang… - Frontiers in Plant …, 2020 - frontiersin.org
Chloroplastic glutamine phosphoribosylpyrophosphate amidotransferase (GPRATase)
catalyzes the first committed step of de novo purine biosynthesis in Arabidopsis thaliana …