False negative rate of COVID-19 PCR testing: a discordant testing analysis

JN Kanji, N Zelyas, C MacDonald, K Pabbaraju… - Virology journal, 2021 - Springer
Background COVID-19 is diagnosed via detection of SARS-CoV-2 RNA using real time
reverse-transcriptase polymerase chain reaction (rtRT-PCR). Performance of many SARS …

Does normalization of SARS-CoV-2 concentrations by Pepper Mild Mottle Virus improve correlations and lead time between wastewater surveillance and clinical data …

R Maal-Bared, Y Qiu, Q Li, T Gao, SE Hrudey… - Science of The Total …, 2023 - Elsevier
Wastewater-based surveillance (WBS) data normalization is an analyte measurement
correction that addresses variations resulting from dilution of fecal discharge by non-sanitary …

Integrating reverse transcription recombinase polymerase amplification with CRISPR technology for the one-tube assay of RNA

W Feng, H Peng, J Xu, Y Liu, K Pabbaraju… - Analytical …, 2021 - ACS Publications
CRISPR-Cas systems integrated with nucleic acid amplification techniques improve both
analytical specificity and sensitivity. We describe here issues and solutions for the …

Detection and quantification of infectious severe acute respiratory coronavirus-2 in diverse clinical and environmental samples

YC Lin, RJ Malott, L Ward, L Kiplagat, K Pabbaraju… - Scientific Reports, 2022 - nature.com
To explore the potential modes of Severe Acute Respiratory Coronavirus-2 (SARS-CoV-2)
transmission, we collected 535 diverse clinical and environmental samples from 75 infected …

[HTML][HTML] The use of drones for the delivery of diagnostic test kits and medical supplies to remote First Nations communities during Covid-19

K Flemons, B Baylis, AZ Khan, AW Kirkpatrick… - American Journal of …, 2022 - Elsevier
Background Health care inequity in remote and rural Indigenous communities often involves
difficulty accessing health care services and supplies. Remotely Piloted Aircraft Systems, or …

[HTML][HTML] Validating and optimizing the method for molecular detection and quantification of SARS-CoV-2 in wastewater

Y Qiu, J Yu, K Pabbaraju, BE Lee, T Gao… - Science of the Total …, 2022 - Elsevier
Wastewater surveillance of SARS-CoV-2 has become a promising tool to estimate
population-level changes in community infections and the prevalence of COVID-19 disease …

Precision response to the rise of the SARS-CoV-2 B. 1.1. 7 variant of concern by combining novel PCR assays and genome sequencing for rapid variant detection and …

N Zelyas, K Pabbaraju, MA Croxen, T Lynch… - Microbiology …, 2021 - Am Soc Microbiol
ABSTRACT SARS-CoV-2 variants of concern (VOCs) have emerged as a global threat to the
COVID-19 pandemic response. We implemented a combined approach to quickly detect …

Saliva collected in universal transport media is an effective, simple and high-volume amenable method to detect SARS-CoV-2

BM Berenger, JM Conly… - Clinical …, 2021 - clinicalmicrobiologyandinfection …
During the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-Co-V-2) pandemic
we have encountered unprecedented testing volumes and constant supply chain …

Patient and ward related risk factors in a multi-ward nosocomial outbreak of COVID-19: Outbreak investigation and matched case–control study

J Leal, HM O'Grady, L Armstrong, D Dixit… - … resistance & infection …, 2023 - Springer
Background Risk factors for nosocomial COVID-19 outbreaks continue to evolve. The aim of
this study was to investigate a multi-ward nosocomial outbreak of COVID-19 between 1st …

Tracking SARS-CoV-2 Omicron lineages using real-time reverse transcriptase PCR assays and prospective comparison with genome sequencing

N Zelyas, K Pabbaraju, MA Croxen, T Lynch… - Scientific Reports, 2023 - nature.com
Omicron has become the dominant SARS-CoV-2 variant globally since December 2021,
with distinct waves being associated with separate Omicron sublineages. Rapid detection of …