Cryogenic electron microscopy and data processing enable the determination of structures
of isolated macromolecules to near-atomic resolution. However, these data do not provide …

Recent advances in cryo-electron microscopy have marked only the beginning of the
potential of this technique. To bring structure into cell biology, the modality of cryo-electron …

Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Most life scientists would agree that understanding how cellular processes work requires
structural knowledge about the macromolecules involved. For example, deciphering the …

Cryogenic electron microscopy (cryo-EM) can reveal the molecular details of biological
processes in their native, cellular environment at atomic resolution. However, few cells are …

The structure of chromatin plays pivotal roles in regulating gene transcription, DNA
replication and repair, and chromosome segregation. This structure, however, remains …

Structures of macromolecules in their native state provide unique unambiguous insights into
their functions. Cryo-electron tomography combined with subtomogram averaging …

Cryo–electron tomography (cryo-ET) is a powerful method to elucidate subcellular
architecture and to structurally analyze biomolecules in situ by subtomogram averaging, yet …

Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and
assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to …