Protein degradation occurs through proteasomal, endosomal, and lysosomal pathways.
Technological advancements have allowed for the determination of protein copy numbers …

Targeted protein degradation (TPD) is an emerging modality for research and therapeutics.
Most TPD approaches harness cellular ubiquitin-dependent proteolytic pathways …

Defining the cellular response to pharmacological agents is critical for understanding the
mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate …

Metabolic stability of proteins plays a vital role in various dedicated cellular processes.
Traditional methods of measuring the metabolic stability are time-consuming and expensive …

The hypoxia-inducible factors (HIF) are transcription factors that activate the adaptive
hypoxic response when oxygen levels are low. The HIF transcriptional program increases …

Partial pluripotent reprogramming can reverse features of aging in mammalian cells, but the
impact on somatic identity and the necessity of individual reprogramming factors remain …

All eukaryotes require intricate protein networks to translate developmental signals into
accurate cell fate decisions. Mutations that disturb interactions between network …

DAXX and ATRX are tumor suppressor proteins that form a histone H3. 3 chaperone
complex and are frequently mutated in cancers with the alternative lengthening of telomeres …

Ubiquitination is a post-translational modification initiated by the E1 enzyme UBA1, which
transfers ubiquitin to~ 35 E2 ubiquitin-conjugating enzymes. While UBA1 loss is cell lethal, it …

Tandem mass tags (TMT) and tribrid mass spectrometers are a powerful combination for
high-throughput proteomics with high quantitative accuracy. Increasingly, this technology is …