There are at least 21 families of enveloped viruses that infect mammals, and many contain
members of high concern for global human health. All enveloped viruses have a dedicated …

The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-
ET resolution revolution. Meanwhile, significant efforts have been made to improve the …

Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study
of viruses ever since its first applications to biological systems. Following the success of …

Highlights•Whole-cell and on-lamellae cryo-ET for structural studies of viral
infection.•Inactivation protocols for improved structural preservation and biosafety.•VLP and …

Since the outbreak of the COVID-19 pandemic, cryo-electron microscopy (cryo-EM) and cryo-
electron tomography (cryo-ET) have been demonstrated to be powerful and efficient tools in …

The coronavirus disease 2019 (COVID-19) pandemic triggered an unprecedented
concentration of economic and research efforts to generate knowledge at unequalled speed …

Before the resolution revolution, cryoelectron microscopy (cryo-EM) single-particle analysis
(SPA) already achieved resolutions beyond 4 Å for certain icosahedral viruses, enabling ab …

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported at the end of
2019 as a worldwide health concern causing a pandemic of unusual viral pneumonia and …

Understanding the molecular properties of SARS-CoV-2 is crucial to tackle future outbreaks.
Current knowledge of the trimeric spike protein relies on truncated recombinant proteins and …

A detergent-free protocol for purification of the coronavirus prefusion S-protein using styrene-
maleic acid copolymer (SMA) was developed. Expression of the S-protein was carried out in …