The applications of nanopore sequencing technology in pathogenic microorganism detection

X Zhu, S Yan, F Yuan, S Wan - Canadian Journal of Infectious …, 2020 - Wiley Online Library
Infectious diseases are major threats to human health and lead to a serious public health
burden. The emergence of new pathogens and the mutation of known pathogens challenge …

Long-reads-based metagenomics in clinical diagnosis with a special focus on fungal infections

MTV Hoang, L Irinyi, Y Hu, B Schwessinger… - Frontiers in …, 2022 - frontiersin.org
Identification of the causative infectious agent is essential in the management of infectious
diseases, with the ideal diagnostic method being rapid, accurate, and informative, while …

The impact of smoking different tobacco types on the subgingival microbiome and periodontal health: A pilot study

S Al Kawas, F Al-Marzooq, B Rahman, JA Shearston… - Scientific reports, 2021 - nature.com
Smoking is a risk factor for periodontal disease, and a cause of oral microbiome dysbiosis.
While this has been evaluated for traditional cigarette smoking, there is limited research on …

Detection of pathogens and antimicrobial resistance genes in ventilator-associated pneumonia by metagenomic next-generation sequencing approach

T Chen, L Zhang, W Huang, H Zong, Q Li… - Infection and Drug …, 2023 - Taylor & Francis
Background The early identification of pathogens and their antibiotic resistance are
essential for the management and treatment of patients affected by ventilator-associated …

Next-and third-generation sequencing outperforms culture-based methods in the diagnosis of ascitic fluid bacterial infections of ICU patients

H Goelz, S Wetzel, N Mehrbarzin, S Utzolino, G Häcker… - Cells, 2021 - mdpi.com
Objectives: Infections of the ascitic fluid are serious conditions that require rapid diagnosis
and treatment. Ascites is often accompanied by other critical pathologies such as …

The application of targeted nanopore sequencing for the identification of pathogens and resistance genes in lower respiratory tract infections

H Zhang, M Wang, X Han, T Wang, Y Lei… - Frontiers in …, 2022 - frontiersin.org
Objectives Lower respiratory tract infections (LRTIs) are one of the causes of mortality
among infectious diseases. Microbial cultures commonly used in clinical practice are time …

Nanopore electrochemistry for pathogen detection

Y Ju, M Pu, K Sun, G Song… - Chemistry–An Asian …, 2022 - Wiley Online Library
Pathogen infections seriously threaten human health, and there is an urgent demand for
rapid and efficient pathogen identification to provide instructions in clinical diagnosis and …

Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections

Q Deng, Y Cao, X Wan, B Wang, A Sun… - Frontiers in Cellular …, 2022 - frontiersin.org
Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt
diagnosis and precise anti-infectives treatment are critical. This study aimed to evaluate the …

Improving the diagnosis of bacterial infections: evaluation of 16S rRNA nanopore metagenomics in culture-negative samples

C Bouchiat, C Ginevra, Y Benito, T Gaillard… - Frontiers in …, 2022 - frontiersin.org
While 16S rRNA PCR-Sanger sequencing has paved the way for the diagnosis of culture-
negative bacterial infections, it does not provide the composition of polymicrobial infections …

Rapid identification of pathogens associated with ventilator-associated pneumonia by Nanopore sequencing

N Wu, P Ranjan, C Tao, C Liu, E Yang, B He… - Respiratory …, 2021 - Springer
Background Aetiology detection is crucial in the diagnosis and treatment of ventilator-
associated pneumonia (VAP). However, the detection method needs improvement. In this …