Liquid Chromatography as a separation tool has made significant strides in a systematic
study. The most widely used chromatographic process is reverse-phase liquid …

A novel mixing unit is proposed for the serial coupling of orthogonal columns to analyze
polar and non-polar compounds in a single run. The principle relies on the isolation of …

Proteins and glycoproteins with therapeutic activity are susceptible to environmental factors,
which can cause their degradation and the loss of their activity. Thus, the maintenance of …

Since their introduction twenty years ago, hybrid particle columns have become widely used
in an ever-expanding range of applications. By combining the desirable features of organic …

Retention behaviour of Dalargin and five peptide analogues of Leu-enkephalin, has been
extensively studied by hydrophilic interaction liquid chromatography (HILIC) on a bare silica …

Hydrophilic interaction chromatography (HILIC) coupled to mass spectrometry (MS) is
considered as the reference analytical technique for glycans profiling, especially for the …

This chapter presents a methodical overview of recent research literature dealing with the
characterization of glycoproteins by hydrophilic interaction chromatography separations …

As I look through the topics that have been discussed in this column over the past 30+ years,
and think about our day-to-day experiences with modern liquid chromatography (LC), it is …

Therapeutic proteins make up a significant proportion of the drugs currently approved or
under clinical development. Most of these proteins are glycosylated, and glycosylation …

Immunoglobulin (Ig) glycosylation has been shown to dramatically affect its structure and
effector functions. Ig glycosylation changes have been associated with different diseases …