Single‐cell RNA sequencing technologies and applications: A brief overview

D Jovic, X Liang, H Zeng, L Lin, F Xu… - Clinical and …, 2022 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

[HTML][HTML] Single‐cell RNA sequencing in cancer research

Y Zhang, D Wang, M Peng, L Tang, J Ouyang… - Journal of Experimental …, 2021 - Springer
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of
complex tissues at single-cell levels, can identify differential gene expression and epigenetic …

CellMarker 2.0: an updated database of manually curated cell markers in human/mouse and web tools based on scRNA-seq data

C Hu, T Li, Y Xu, X Zhang, F Li, J Bai… - Nucleic Acids …, 2023 - academic.oup.com
Abstract CellMarker 2.0 (http://bio-bigdata. hrbmu. edu. cn/CellMarker or http://117.50.
127.228/CellMarker/) is an updated database that provides a manually curated collection of …

Beyond bulk: a review of single cell transcriptomics methodologies and applications

A Kulkarni, AG Anderson, DP Merullo… - Current opinion in …, 2019 - Elsevier
Highlights•Technologies such as single-cell RNA sequencing have rapidly progressed in
recent years.•Various methods exist for preparing libraries for sequencing, each with its own …

[HTML][HTML] Prognostic and predictive enrichment in sepsis

NL Stanski, HR Wong - Nature Reviews Nephrology, 2020 - nature.com
Sepsis is a heterogeneous disease state that is both common and consequential in critically
ill patients. Unfortunately, the heterogeneity of sepsis at the individual patient level has …

Single-cell RNA sequencing analysis: a step-by-step overview

S Slovin, A Carissimo, F Panariello, A Grimaldi… - RNA …, 2021 - Springer
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Single‐cell RNA sequencing in breast cancer: Understanding tumor heterogeneity and paving roads to individualized therapy

S Ding, X Chen, K Shen - Cancer Communications, 2020 - Wiley Online Library
Single‐cell RNA sequencing (scRNA‐seq) is a novel technology that allows transcriptomic
analyses of individual cells. During the past decade, scRNA‐seq sensitivity, accuracy, and …

[PDF][PDF] Unravelling intratumoral heterogeneity through high-sensitivity single-cell mutational analysis and parallel RNA sequencing

A Rodriguez-Meira, G Buck, SA Clark, BJ Povinelli… - Molecular cell, 2019 - cell.com
Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for resolving
transcriptional heterogeneity. However, its application to studying cancerous tissues is …

[HTML][HTML] Integrating single-cell analysis and machine learning to create glycosylation-based gene signature for prognostic prediction of uveal melanoma

J Liu, P Zhang, F Yang, K Jiang, S Sun, Z Xia… - Frontiers in …, 2023 - frontiersin.org
Background Increasing evidence suggests a correlation between glycosylation and the
onset of cancer. However, the clinical relevance of glycosylation-related genes (GRGs) in …

[HTML][HTML] A comparison for dimensionality reduction methods of single-cell RNA-seq data

R Xiang, W Wang, L Yang, S Wang, C Xu… - Frontiers in genetics, 2021 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) is a high-throughput sequencing technology
performed at the level of an individual cell, which can have a potential to understand cellular …