Transformation-associated recombination (TAR) cloning represents a unique tool for
isolation and manipulation of large DNA molecules. The technique exploits a high level of …

The structural and functional analysis of mammalian genomes would benefit from the ability
to isolate from multiple DNA samples any targeted chromosomal segment that is the size of …

Despite the ever-increasing output of Illumina sequencing data, loci with extreme base
compositions are often under-represented or absent. To evaluate sources of base …

Here, we describe a protocol for the selective isolation of any genomic fragment or gene of
interest up to 250 kb in size from complex genomes as a circular yeast artificial chromosome …

An understanding of how centromeric transition regions are organized is a critical aspect of
chromosome structure and function; however, the sequence context of these regions has …

Abstract Background Tandemly Repeated DNA represents a large portion of the human
genome, and accounts for a significant amount of copy number variation. Here we present a …

Despite the key role of the human ribosome in protein biosynthesis, little is known about the
extent of sequence variation in ribosomal DNA (rDNA) or its pre-rRNA and rRNA products …

In vivo expression of human telomerase is significantly different from that of mouse
telomerase. To assess the basis for this difference, a bacterial artificial chromosome clone …

Repetitive DNA is widespread in eukaryotic genomes, in some cases making up more than
80% of the total. SSRs are a type of repetitive DNA formed by short motifs repeated in …

Here we describe an updated TAR cloning protocol for the selective and efficient isolation of
any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The …