The maturation of high-throughput short-read sequencing technology over the past two
decades has shaped the way genomes are studied. Recently, single-molecule, long-read …

Spatial omics emerged as a new frontier of biological and biomedical research. Here, we
present spatial-CUT&Tag for spatially resolved genome-wide profiling of histone …

Transcription factors (TFs) orchestrate the gene expression programs that define each cell's
identity. The canonical TF accomplishes this with two domains, one that binds specific DNA …

The role of histone modifications in transcription remains incompletely understood. Here, we
examine the relationship between histone modifications and transcription using …

Recent efforts have succeeded in surveying open chromatin at the single-cell level, but high-
throughput, single-cell assessment of heterochromatin and its underlying genomic …

Highly coordinated changes in gene expression underlie T cell activation and exhaustion.
However, the mechanisms by which such programs are regulated and how these may be …

It has been just over 10 years since the initial description of transposase-based methods to
prepare high-throughput sequencing libraries, or “tagmentation,” in which a hyperactive …

Chromatin accessibility is a hallmark of active transcription and entails ATP-dependent
nucleosome remodeling, which is carried out by complexes such as Brahma-associated …

ATRX is a tumor suppressor that has been associated with protection from DNA replication
stress, purportedly through resolution of difficult-to-replicate G-quadruplex (G4) DNA …

Abstract Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed
transposase tethering strategy for in situ chromatin profiling in small samples and single …