The extremely low efficiency of human embryonic stem cell (hESC) derivation using somatic
cell nuclear transfer (SCNT) limits its potential application. Blastocyst formation from human …

Background Previously, a role was demonstrated for transcription in the acquisition of DNA
methylation at imprinted control regions in oocytes. Definition of the oocyte DNA methylome …

Motivation Single-cell RNA-sequencing (scRNA-seq) technology provides a powerful tool for
investigating cell heterogeneity and cell subpopulations by allowing the quantification of …

Active DNA demethylation in mammals involves ten-eleven translocation (TET) family
protein-mediated oxidation of 5-methylcytosine (5mC). However, base-resolution …

The causes of embryonic arrest during pre-implantation development are poorly understood.
Attempts to correlate patterns of oocyte gene expression with successful embryo …

Cell-to-cell variation and heterogeneity are fundamental and intrinsic characteristics of stem
cell populations, but these differences are masked when bulk cells are used for omic …

There is surprising confusion surrounding the concept of biological totipotency, both within
the scientific community and in society at large. Increasingly, ethical objections to scientific …

Cell types are the basic building units of multicellular life, with extensive diversities. The
evolution of cell types is a crucial layer of comparative cell biology but is thus far not …

This study provides an assessment of the Fluidigm C1 platform for RNA sequencing of
single mouse pancreatic islet cells. The system combines microfluidic technology and …

With the advancement of human stem cell cultures and interest in understanding human
embryogenesis, human blastocyst-like structures, or human blastoids, are being developed …