Recent advances and challenges of RT-PCR tests for the diagnosis of COVID-19

M Teymouri, S Mollazadeh, H Mortazavi… - … -Research and Practice, 2021 - Elsevier
Since the outbreak of the novel severe acute respiratory syndrome coronavirus-2 (SARS-
CoV-2), the control of virus spread has remained challenging given the pitfalls of the current …

RNA-extraction-free nano-amplified colorimetric test for point-of-care clinical diagnosis of COVID-19

M Alafeef, P Moitra, K Dighe, D Pan - Nature Protocols, 2021 - nature.com
The global pandemic of coronavirus disease 2019 (COVID-19) highlights the shortcomings
of the current testing paradigm for viral disease diagnostics. Here, we report a stepwise …

CRISPR systems for COVID-19 diagnosis

H Rahimi, M Salehiabar, M Barsbay, M Ghaffarlou… - ACS …, 2021 - ACS Publications
The emergence of the new coronavirus 2019 (COVID-19) was first seen in December 2019,
which has spread rapidly and become a global pandemic. The number of cases of COVID …

Point of care detection of COVID-19: Advancement in biosensing and diagnostic methods

S Suleman, SK Shukla, N Malhotra… - Chemical Engineering …, 2021 - Elsevier
The recent outbreak of COVID-19 has created much inconvenience and fear that the virus
can seriously affect humans, causing health hazards and death. This pandemic has created …

Advanced molecular and immunological diagnostic methods to detect SARS-CoV-2 infection

JC Rotondo, F Martini, M Maritati, E Caselli… - Microorganisms, 2022 - mdpi.com
Simple Summary COVID-19 emerged in late 2019 in China and rapidly spread across the
globe. After 2 years, numerous advances have been made. First of all, the preventive …

Preanalytical issues and cycle threshold values in SARS-CoV-2 real-time RT-PCR testing: should test results include these?

I Engelmann, EK Alidjinou, J Ogiez, Q Pagneux… - ACS …, 2021 - ACS Publications
Since the emergence of SARS-CoV-2 pandemic, clinical laboratories worldwide are
overwhelmed with SARS-CoV-2 testing using the current gold standard: real-time reverse …

Advancement in COVID‐19 detection using nanomaterial‐based biosensors

PL Truong, Y Yin, D Lee, SH Ko - Exploration, 2023 - Wiley Online Library
Abstract Coronavirus disease 2019 (COVID‐19) pandemic has exemplified how viral growth
and transmission are a significant threat to global biosecurity. The early detection and …

Performance of a point-of-care test for the rapid detection of SARS-CoV-2 antigen

A Strömer, R Rose, M Schäfer, F Schön, A Vollersen… - Microorganisms, 2020 - mdpi.com
The rapid detection of infections caused by the severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2) is necessary in the ongoing pandemic. Antigen-specific point …

Detect and destroy: CRISPR-based technologies for the response against viruses

CA Freije, PC Sabeti - Cell host & microbe, 2021 - cell.com
Despite numerous viral outbreaks in the last decade, including a devastating global
pandemic, diagnostic and therapeutic technologies remain severely lacking. CRISPR-Cas …

FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip

M Kumar, S Gulati, AH Ansari, R Phutela, S Acharya… - Elife, 2021 - elifesciences.org
The COVID-19 pandemic originating in the Wuhan province of China in late 2019 has
impacted global health, causing increased mortality among elderly patients and individuals …