Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

Adipose tissue exhibits remarkable plasticity with capacity to change in size and cellular
composition under physiological and pathophysiological conditions. The emergence of …

Single-cell technologies have transformed our understanding of human tissues. Yet, studies
typically capture only a limited number of donors and disagree on cell type definitions …

Inferring single-cell compositions and their contributions to global gene expression changes
from bulk RNA sequencing (RNA-seq) datasets is a major challenge in oncology. Here we …

A key aspect of nearly all single-cell sequencing experiments is dissociation of intact tissues
into single-cell suspensions. While many protocols have been optimized for optimal cell …

Understanding gene function and regulation in homeostasis and disease requires
knowledge of the cellular and tissue contexts in which genes are expressed. Here, we …

Loss of skeletal integrity during ageing and disease is associated with an imbalance in the
opposing actions of osteoblasts and osteoclasts. Here we show that intrinsic ageing of …

Adipose tissues display a remarkable ability to adapt to the dietary status. Here, we have
applied single-nucleus RNA-seq to map the plasticity of mouse epididymal white adipose …

Differential expression analysis in single-cell transcriptomics enables the dissection of cell-
type-specific responses to perturbations such as disease, trauma, or experimental …

Human brain development is underpinned by cellular and molecular reconfigurations
continuing into the third decade of life. To reveal cell dynamics orchestrating neural …