Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

The ability of chemical modifications of single nucleotides to alter the electrostatic charge,
hydrophobic surface and base pairing of RNA molecules is exploited for the clinical use of …

Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early
adopters have used this technology to document nucleotide modifications and 3 …

Transfer RNAs (tRNAs) play a central role in protein translation. Studying them has been
difficult in part because a simple method to simultaneously quantify their abundance and …

Over the past decade, long-read, single-molecule DNA sequencing technologies have
emerged as powerful players in genomics. With the ability to generate reads tens to …

RNA modifications, such as N 6-methyladenosine (m6A), modulate functions of cellular RNA
species. However, quantifying differences in RNA modifications has been challenging. Here …

Nanopore RNA sequencing shows promise as a method for discriminating and identifying
different RNA modifications in native RNA. Expanding on the ability of nanopore sequencing …

Abstract N6-methyladenosine (m6A) has been increasingly recognized as a new and
important regulator of gene expression. To date, transcriptome-wide m6A detection primarily …

RNA modifications such as m6A methylation form an additional layer of complexity in the
transcriptome. Nanopore direct RNA sequencing can capture this information in the raw …

Long-read sequencing (LRS) technologies have provided extremely powerful tools to
explore genomes. While in the early years these methods suffered technical limitations, they …