Optical tweezers in single-molecule biophysics
CJ Bustamante, YR Chemla, S Liu… - Nature Reviews Methods …, 2021 - nature.com
Optical tweezers have become the method of choice in single-molecule manipulation
studies. In this Primer, we first review the physical principles of optical tweezers and the …
studies. In this Primer, we first review the physical principles of optical tweezers and the …
Single-molecule analysis and engineering of DNA motors
Molecular motors are diverse enzymes that transduce chemical energy into mechanical
work and, in doing so, perform critical cellular functions such as DNA replication and …
work and, in doing so, perform critical cellular functions such as DNA replication and …
The post-PAM interaction of RNA-guided spCas9 with DNA dictates its target binding and dissociation
Cas9 is an RNA-guided endonuclease that targets complementary DNA for cleavage and
has been repurposed for many biological usages. Cas9 activities are governed by its direct …
has been repurposed for many biological usages. Cas9 activities are governed by its direct …
Dynamics of Staphylococcus aureus Cas9 in DNA target Association and Dissociation
Abstract Staphylococcus aureus Cas9 (SaCas9) is an RNA‐guided endonuclease that
targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its …
targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its …
Non-homologous end-joining at challenged replication forks: an RNA connection?
C Audoynaud, S Vagner, S Lambert - Trends in Genetics, 2021 - cell.com
Defective DNA replication, known as 'replication stress', is a source of DNA damage, a
hallmark of numerous human diseases, including cancer, developmental defect …
hallmark of numerous human diseases, including cancer, developmental defect …
DNA helicase–polymerase coupling in bacteriophage DNA replication
Bacteriophages have long been model systems to study the molecular mechanisms of DNA
replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively …
replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively …
Towards biological applications of nanophotonic tweezers
Optical trapping (synonymous with optical tweezers) has become a core biophysical
technique widely used for interrogating fundamental biological processes on size scales …
technique widely used for interrogating fundamental biological processes on size scales …
ALT control, delete: FANCM as an anti-cancer target in Alternative Lengthening of Telomeres
Break-induced replication is a specific type of DNA repair that has a co-opted role in
telomere extension by telomerase-negative cancer cells. This Alternative Lengthening of …
telomere extension by telomerase-negative cancer cells. This Alternative Lengthening of …
Joint Efforts of Replicative Helicase and SSB Ensure Inherent Replicative Tolerance of G‐Quadruplex
L Guo, Y Bao, Y Zhao, Z Ren, L Bi, X Zhang… - Advanced …, 2024 - Wiley Online Library
Abstract G‐quadruplex (G4) is a four‐stranded noncanonical DNA structure that has long
been recognized as a potential hindrance to DNA replication. However, how replisomes …
been recognized as a potential hindrance to DNA replication. However, how replisomes …
Single-molecule insights into the dynamics of replicative helicases
Helicases are molecular motors that translocate along single-stranded DNA and unwind
duplex DNA. They rely on the consumption of chemical energy from nucleotide hydrolysis to …
duplex DNA. They rely on the consumption of chemical energy from nucleotide hydrolysis to …