Production and purification of bacterial membrane vesicles for biotechnology applications: Challenges and opportunities

KF Castillo‐Romero, A Santacruz… - …, 2023 - Wiley Online Library
Bacterial membrane vesicles (BMVs) are bi‐layered nanostructures derived from Gram‐
negative and Gram‐positive bacteria. Among other pathophysiological roles, BMVs are …

Deciphering psychobiotics' mechanism of action: bacterial extracellular vesicles in the spotlight

L Bleibel, S Dziomba, KF Waleron… - Frontiers in …, 2023 - frontiersin.org
The intake of psychobiotic bacteria appears to be a promising adjunct to neuropsychiatric
treatment, and their consumption may even be beneficial for healthy people in terms of …

The role of extracellular vesicles in periodontitis: pathogenesis, diagnosis, and therapy

R Cai, L Wang, W Zhang, B Liu, Y Wu, J Pang… - Frontiers in …, 2023 - frontiersin.org
Periodontitis is a prevalent disease and one of the leading causes of tooth loss. Biofilms are
initiating factor of periodontitis, which can destroy periodontal tissue by producing virulence …

Assessment of small cellular particles from four different natural sources and liposomes by interferometric light microscopy

A Romolo, Z Jan, A Bedina Zavec, M Kisovec… - International journal of …, 2022 - mdpi.com
Small particles in natural sources are a subject of interest for their potential role in
intercellular, inter-organism, and inter-species interactions, but their harvesting and …

Isolation of Citrus lemon extracellular vesicles: Development and process control using capillary electrophoresis

A Steć, M Chodkowska, J Kasprzyk-Pochopień… - Food Chemistry, 2023 - Elsevier
A new and scalable method for the isolation of extracellular vesicles (EV) from Citrus lemon
juice samples was developed. The methodology included preliminary preconcentration of …

Biological functions and biomedical applications of extracellular vesicles derived from blood cells

K Pan, Y Zhu, P Chen, K Yang, Y Chen, Y Wang… - Free Radical Biology …, 2024 - Elsevier
There is a growing interest in using extracellular vesicles (EVs) for therapeutic applications.
EVs are composed of cytoplasmic proteins and nucleic acids and an external lipid bilayer …

Protein overproduction alters exosome secretion in Chinese hamster ovary cells

A Steć, M Targońska, E Karkosińska, M Słowik… - Analytical and …, 2023 - Springer
Despite the abundance of available cell lines, nearly 70% of all recombinant therapeutic
proteins today are produced in Chinese hamster ovary (CHO) cells. The impact of protein …

[HTML][HTML] Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform

A Steć, A Heinz, S Dziomba - Journal of Pharmaceutical Analysis, 2024 - Elsevier
Extracellular vesicles (EVs) are a part of a cell-to-cell communication system of prokaryotic
and eukaryotic organisms. Their ability to penetrate biological barriers and to transfer …

Extracellular Vesicles in Microbes, Pathogens, and Infectious Diseases

FWN Chow, RM Morphew - International Journal of Molecular Sciences, 2023 - mdpi.com
Extracellular vesicles (EVs) are nanosized lipid bilayer particles that are produced by all
kinds of organisms, including both pathogenic and non-pathogenic archaea, bacteria, fungi …

Membrane Vesicles of Clostridioides difficile and Other Clostridial Species

S Goh, J Inal - Updates on Clostridioides difficile in Europe: Advances …, 2024 - Springer
Membrane vesicles are secreted by growing bacterial cells and are important components of
the bacterial secretome, with a role in delivering effector molecules that ultimately enable …