Mass spectrometry‐based high‐throughput proteomics and its role in biomedical studies and systems biology

CB Messner, V Demichev, Z Wang, J Hartl… - …, 2023 - Wiley Online Library
There are multiple reasons why the next generation of biological and medical studies
require increasing numbers of samples. Biological systems are dynamic, and the effect of a …

Ultra-fast label-free quantification and comprehensive proteome coverage with narrow-window data-independent acquisition

UH Guzman, A Martinez-Val, Z Ye, E Damoc… - Nature …, 2024 - nature.com
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes
in a fast and reproducible manner. Here we present the narrow-window data-independent …

Instrumentation at the leading edge of proteomics

TM Peters-Clarke, JJ Coon, NM Riley - Analytical Chemistry, 2024 - ACS Publications
The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …

Metabolic heterogeneity and cross-feeding within isogenic yeast populations captured by DILAC

S Kamrad, C Correia-Melo, L Szyrwiel, SK Aulakh… - Nature …, 2023 - nature.com
Genetically identical cells are known to differ in many physiological parameters such as
growth rate and drug tolerance. Metabolic specialization is believed to be a cause of such …

Narrow-window DIA: Ultra-fast quantitative analysis of comprehensive proteomes with high sequencing depth

UH Guzman, A Martinez Del Val, Z Ye, E Damoc… - BioRxiv, 2023 - biorxiv.org
Mass spectrometry (MS)-based proteomics aims to characterize comprehensive proteomes
in a fast and reproducible manner. Here, we present an ultra-fast scanning data …

Slice-PASEF: fragmenting all ions for maximum sensitivity in proteomics

L Szyrwiel, L Sinn, M Ralser, V Demichev - BioRxiv, 2022 - biorxiv.org
We present Slice-PASEF, a novel mass spectrometry technology based on trapped ion
mobility separation of ions. Slice-PASEF allows to achieve the theoretical maximum of …

Fast proteomics with dia‐PASEF and analytical flow‐rate chromatography

L Szyrwiel, C Gille, M Mülleder, V Demichev… - …, 2024 - Wiley Online Library
Increased throughput in proteomic experiments can improve accessibility of proteomic
platforms, reduce costs, and facilitate new approaches in systems biology and biomedical …

Natural proteome diversity links aneuploidy tolerance to protein turnover

J Muenzner, P Trébulle, F Agostini, H Zauber… - Nature, 2024 - nature.com
Accessing the natural genetic diversity of species unveils hidden genetic traits, clarifies gene
functions and allows the generalizability of laboratory findings to be assessed. One notable …

Protein translation: biological processes and therapeutic strategies for human diseases

X Jia, X He, C Huang, J Li, Z Dong, K Liu - Signal Transduction and …, 2024 - nature.com
Protein translation is a tightly regulated cellular process that is essential for gene expression
and protein synthesis. The deregulation of this process is increasingly recognized as a …

Redefining modern food analysis: significance of omics analytical techniques integration, chemometrics and bioinformatics

CO Okoye, H Jiang, M Nazar, XL Tan, J Jiang - TrAC Trends in Analytical …, 2024 - Elsevier
Food analysis is a scientific discipline that has evolved from traditional methods into
sophisticated approaches due to advances in analytical chemistry and modern …