Challenges and opportunities for global genomic surveillance strategies in the COVID-19 era

T Ling-Hu, E Rios-Guzman, R Lorenzo-Redondo… - Viruses, 2022 - mdpi.com
Global SARS-CoV-2 genomic surveillance efforts have provided critical data on the ongoing
evolution of the virus to inform best practices in clinical care and public health throughout the …

A case for investment in clinical metagenomics in low-income and middle-income countries

G Marais, D Hardie, A Brink - The Lancet Microbe, 2023 - thelancet.com
Clinical metagenomics is the diagnostic approach with the broadest capacity to detect both
known and novel pathogens. Clinical metagenomics is costly to run and requires …

Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing

T Cha, HH Kim, J Keum, MJ Kwak, JY Park… - Frontiers in …, 2023 - frontiersin.org
This study aimed to evaluate the difference in gut microbiomes between preterm and term
infants using third-generation long-read sequencing (Oxford Nanopore Technologies, ONT) …

Comparison of Nanopore and Synthesis-Based Next-Generation Sequencing Platforms for SARS-CoV-2 Variant Monitoring in Wastewater

D Garcia-Pedemonte, A Carcereny, J Gregori… - International Journal of …, 2023 - mdpi.com
Shortly after the beginning of the SARS-CoV-2 pandemic, many countries implemented
sewage sentinel systems to monitor the circulation of the virus in the population. A …

De novo assembled mitogenome analysis of Trichuris trichiura from Korean individuals using nanopore-based long-read sequencing technology

JO Delaluna, H Kang, YY Chang, MJ Kim… - PLoS neglected …, 2023 - journals.plos.org
Knowledge about mitogenomes has been proven to be essential in human parasite
diagnostics and understanding of their diversity. However, the lack of substantial data for …

Technical comparison of MinIon and Illumina technologies for genotyping Chikungunya virus in clinical samples

LM De Souza, ID De Oliveira, FCS Sales… - Journal of Genetic …, 2023 - Springer
New-generation sequencing (NGS) techniques have brought the opportunity for genomic
monitoring of several microorganisms potentially relevant to public health. The …

Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections

Q Deng, Y Cao, X Wan, B Wang, A Sun… - Frontiers in Cellular …, 2022 - frontiersin.org
Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt
diagnosis and precise anti-infectives treatment are critical. This study aimed to evaluate the …

Expedited retrieval of high-quality Usutu virus genomes via Nanopore sequencing with and without target enrichment

CM Holicki, F Bergmann, F Stoek, A Schulz… - Frontiers in …, 2022 - frontiersin.org
Usutu virus (USUV) is a mosquito-borne zoonotic virus and one of the causes of flavivirus
encephalitis in birds and occasionally in humans. USUV rapidly disperses in a susceptible …

Bioprotective lactobacilli in Crescenza and Gouda cheese models to inhibit fungal spoilage

Z Zhao, DJ Simpson, MG Gänzle - International Dairy Journal, 2024 - Elsevier
Bioprotective cultures are applied to control the fungal spoilage of fermented dairy products
but only few studies evaluated their efficacy in cheese. To evaluate growth and antifungal …

Assessing microbial diversity in Yellowstone National Park hot springs using a field deployable automated nucleic acid extraction system

JM Wood, C Urbaniak, C Parker, NK Singh… - Frontiers in Ecology …, 2024 - frontiersin.org
Microbial diversity estimation involves extracting nucleic acids from intricate sample
matrices. Preparing nucleic acid samples is time-consuming, necessitating effective cell lysis …