Single-cell biology is facing a crisis of sorts. Vast numbers of single-cell molecular profiles
are being generated, clustered and annotated. However, this is overwhelmingly ad hoc, and …

Central to the core principle of cell theory, depicting cells' history, state and fate is a
fundamental goal in modern biology. By leveraging clonal analysis and single-cell RNA-seq …

In vitro cultured stem cells with distinct developmental capacities can contribute to
embryonic or extraembryonic tissues after microinjection into pre-implantation mammalian …

Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …

Self-organizing neural organoids grown from pluripotent stem cells,–combined with single-
cell genomic technologies provide opportunities to examine gene regulatory networks …

Human brain development is underpinned by cellular and molecular reconfigurations
continuing into the third decade of life. To reveal cell dynamics orchestrating neural …

Several in vitro models have been developed to recapitulate mouse embryogenesis solely
from embryonic stem cells (ESCs). Despite mimicking many aspects of early development …

The house mouse (Mus musculus) is an exceptional model system, combining genetic
tractability with close evolutionary affinity to humans,. Mouse gestation lasts only 3 weeks …

Our understanding of human early development is severely hampered by limited access to
embryonic tissues. Due to their close evolutionary relationship with humans, nonhuman …

Waddington's epigenetic landscape is a metaphor frequently used to illustrate cell
differentiation. Recent advances in single-cell genomics are altering our understanding of …