Serial Lift-Out: sampling the molecular anatomy of whole organisms
Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …
MicroED in drug discovery
The cryo-electron microscopy (cryo-EM) method microcrystal electron diffraction (MicroED)
was initially described in 2013 and has recently gained attention as an emerging technique …
was initially described in 2013 and has recently gained attention as an emerging technique …
Applying 3D ED/MicroED workflows toward the next frontiers
M Aragon, SEJ Bowman, CH Chen… - … Section C: Structural …, 2024 - journals.iucr.org
We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED),
as discussed during a symposium at the National Center for CryoEM Access and Training …
as discussed during a symposium at the National Center for CryoEM Access and Training …
Cryo-plasma FIB/SEM volume imaging of biological specimens
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and
assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to …
assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to …
The reduction of FIB damage on cryo-lamella by lowering energy of ion beam revealed by a quantitative analysis
Focused ion beam (FIB) is widely used for thinning frozen cells to produce lamellae for cryo-
electron microscopy imaging and for protein structures study in vivo. However, FIB damages …
electron microscopy imaging and for protein structures study in vivo. However, FIB damages …
Making the Most of 3D Electron Diffraction: Best Practices to Handle a New Tool
Along with the adoption of three-dimensional electron diffraction (3D ED/MicroED) as a
mainstream tool for structure determination from sub-micron single crystals, questions about …
mainstream tool for structure determination from sub-micron single crystals, questions about …
[HTML][HTML] Design and implementation of suspended drop crystallization
In this work, a novel crystal growth method termed suspended drop crystallization has been
developed. Unlike traditional methods, this technique involves mixing protein and precipitant …
developed. Unlike traditional methods, this technique involves mixing protein and precipitant …
Electron counting with direct electron detectors in MicroED
The combination of high sensitivity and rapid readout makes it possible for electron-counting
detectors to record cryogenic electron microscopy data faster and more accurately without …
detectors to record cryogenic electron microscopy data faster and more accurately without …
3D Nanocrystallography and the Imperfect Molecular Lattice
Crystallographic analysis relies on the scattering of quanta from arrays of atoms that
populate a repeating lattice. While large crystals built of lattices that appear ideal are sought …
populate a repeating lattice. While large crystals built of lattices that appear ideal are sought …
Scaling up cryo-EM for biology and chemistry: The journey from niche technology to mainstream method
MJ de la Cruz, ET Eng - Structure, 2023 - cell.com
Cryoelectron microscopy (cryo-EM) methods have made meaningful contributions in a wide
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …
variety of scientific research fields. In structural biology, cryo-EM routinely elucidates …