Second harmonic generation microscopy: a powerful tool for bio-imaging

A Aghigh, S Bancelin, M Rivard, M Pinsard… - Biophysical …, 2023 - Springer
Second harmonic generation (SHG) microscopy is an important optical imaging technique in
a variety of applications. This article describes the history and physical principles of SHG …

Changes of collagen ultrastructure in breast cancer tissue determined by second-harmonic generation double Stokes-Mueller polarimetric microscopy

A Golaraei, L Kontenis, R Cisek, D Tokarz… - Biomedical optics …, 2016 - opg.optica.org
Second-harmonic generation (SHG) double Stokes-Mueller polarimetric microscopy is
applied to study the alteration of collagen ultrastructure in a tissue microarray containing …

Multiscale label-free imaging of fibrillar collagen in the tumor microenvironment

MS Nelson, Y Liu, HM Wilson, B Li… - … : Methods and Protocols, 2023 - Springer
With recent advances in cancer therapeutics, there is a great need for improved imaging
methods for characterizing cancer onset and progression in a quantitative and actionable …

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

A Golaraei, K Mirsanaye, Y Ro, S Krouglov… - Journal of …, 2019 - Wiley Online Library
Polarization‐dependent second‐harmonic generation (P‐SHG) microscopy is used to
characterize molecular nonlinear optical properties of collagen and determine a three …

Complex susceptibilities and chiroptical effects of collagen measured with polarimetric second-harmonic generation microscopy

A Golaraei, L Kontenis, K Mirsanaye, S Krouglov… - Scientific reports, 2019 - nature.com
Nonlinear optical properties of collagen type-I are investigated in thin tissue sections of pig
tendon as a research model using a complete polarimetric second-harmonic generation (P …

Wide‐field Stokes polarimetric microscopy for second harmonic generation imaging

L Uribe Castaño, K Mirsanaye, L Kontenis… - Journal of …, 2023 - Wiley Online Library
We employ wide‐field second harmonic generation (SHG) microscopy together with
nonlinear Stokes polarimetry for quick ultrastructural investigation of large sample areas …

Quantifying external and internal collagen organization from Stokes-vector-based second harmonic generation imaging polarimetry

FJ Ávila, O Del Barco, JM Bueno - Journal of Optics, 2017 - iopscience.iop.org
Collagen organization has been analyzed at both external and internal scales by combining
Stokes-vector polarimetry and second harmonic generation microscopy. A significant linear …

Modeling and predicting second harmonic generation from protein molecular structure

B Asadipour, E Beaurepaire, X Zhang, A Chessel… - Biophysical …, 2024 - cell.com
Polarization resolved second-harmonic-generation (pSHG) microscopy is increasingly used
for mapping organized arrays of non-centrosymmetric proteins such as collagen, myosin …

Optimal nonlinear Stokes–Mueller polarimetry for multi-photon processes

X Li, W Liu, F Goudail, SC Chen - Optics Letters, 2022 - opg.optica.org
In this Letter, we present an optimization model for nonlinear Stokes–Mueller polarimetry
(SMP) to improve the precision in estimating the nonlinear Mueller matrix (MM) for two-and …

Second harmonic generation theory for a helical macromolecule with high sensitivity to structural disorder

D Abramavicius, S Krouglov, V Barzda - Physical Chemistry Chemical …, 2021 - pubs.rsc.org
Microscopic theory for the second harmonic generation in a helical molecular system is
developed in the minimal coupling representation including non-local interaction effects. At …