Tier‐1 assays for assessing the toxicity of insecticidal proteins produced by genetically engineered plants to non‐target arthropods

YH Li, J Romeis, KM Wu, YF Peng - Insect Science, 2014 - Wiley Online Library
In assessing an insect‐resistant genetically engineered (IRGE) crop before its
commercialization, researchers normally use so‐called “Tier‐1 assays” as the initial step to …

Selection of reference genes for RT-qPCR analysis in a predatory biological control agent, Coleomegilla maculata (Coleoptera: Coccinellidae)

C Yang, H Pan, JE Noland, D Zhang, Z Zhang, Y Liu… - Scientific reports, 2015 - nature.com
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying gene expression across various biological processes, of which …

A Comprehensive Assessment of the Effects of Bt Cotton on Coleomegilla maculata Demonstrates No Detrimental Effects by Cry1Ac and Cry2Ab

Y Li, J Romeis, P Wang, Y Peng, AM Shelton - PLoS One, 2011 - journals.plos.org
The ladybird beetle, Coleomegilla maculata (DeGeer), is a common and abundant predator
in many cropping systems. Its larvae and adults are predaceous, feeding on aphids, thrips …

Consumption of Bt rice pollen expressing Cry2Aa does not cause adverse effects on adult Chrysoperla sinica Tjeder (Neuroptera: Chrysopidae)

Y Wang, Y Li, J Romeis, X Chen, J Zhang, H Chen… - Biological Control, 2012 - Elsevier
Adults of Chrysoperla sinica Tjeder are prevalent pollen feeders in rice fields in China. They
are therefore exposed to insecticidal proteins when foraging insect-resistant genetically …

Bt crops benefit natural enemies to control non-target pests

JC Tian, J Yao, LP Long, J Romeis, AM Shelton - Scientific reports, 2015 - nature.com
Crops producing insecticidal crystal (Cry) proteins from Bacillus thuringiensis (Bt) control
important lepidopteran pests. However, pests such as aphids not susceptible to Cry proteins …

Use of a Pollen-Based Diet to Expose the Ladybird Beetle Propylea japonica to Insecticidal Proteins

X Zhang, Y Li, J Romeis, X Yin, K Wu, Y Peng - PloS one, 2014 - journals.plos.org
A rape seed pollen-based diet was developed and found to be suitable for use in a dietary
exposure assay for Propylea japonica. Using the diet, we established and validated a …

Characterization of the Spectrum of Activity of IPD079Ea: A Protein Derived From Ophioglossum pendulum (Ophioglossales: Ophioglossaceae) With Activity Against Western …

CJ Boeckman, S Ballou, T Gunderson… - Journal of Economic …, 2022 - academic.oup.com
Western corn rootworm (Diabrotica virgifera virgifera LeConte) is a major pest of corn in both
North America and Europe and as such presents significant challenges for farmers …

Use of an artificial diet system to study the toxicity of gut-active insecticidal compounds on larvae of the green lacewing Chrysoperla sinica

Y Li, L Hu, J Romeis, Y Wang, L Han, X Chen, Y Peng - Biological Control, 2014 - Elsevier
A semi-liquid artificial diet was established and found to be a suitable food source for
Chrysoperla sinica larvae, comparable to aphid prey. Using the artificial diet, we established …

Toxicological and biochemical analyses demonstrate no toxic effect of Cry1C and Cry2A to Folsomia candida

Y Yang, X Chen, L Cheng, F Cao, J Romeis, Y Li… - Scientific Reports, 2015 - nature.com
Collembolans are common soil arthropods that may be exposed to insecticidal proteins
produced in genetically engineered (GE) plants by ingestion of crop residues or root …

Bt rice expressing Cry2Aa does not cause direct detrimental effects on larvae of Chrysoperla sinica

Y Li, Y Wang, J Romeis, Q Liu, K Lin, X Chen, Y Peng - Ecotoxicology, 2013 - Springer
To assess the potential effects of Cry2Aa-expressing insect-resistant Bt rice on Chrysoperla
sinica larvae, we conducted two tritrophic bioassays using a non-target (Laodelphax …