Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of
microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly …

Molecular biology has been led by various measurement technologies, and increased
throughput has developed omics analysis. The development of massively parallel …

Background Species-level genetic characterization of complex bacterial communities has
important clinical applications in both diagnosis and treatment. Amplicon sequencing of the …

Since its introduction, nanopore sequencing has enhanced our ability to study complex
microbial samples through the possibility to sequence long reads in real time using …

Assessment of bacterial diversity through sequencing of 16S ribosomal RNA (16S rRNA)
genes has been an approach widely used in environmental microbiology, particularly since …

Metagenomic sequence data from defined mock communities is crucial for the assessment
of sequencing platform performance and downstream analyses, including assembly, binning …

Illumina and nanopore sequencing technologies are powerful tools that can be used to
determine the bacterial composition of complex microbial communities. In this study, we …

Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …

Background: Profiling the microbiome of low-biomass samples is challenging for
metagenomics since these samples are prone to contain DNA from other sources (eg host or …

Background Prosthetic joint infections are clinically difficult to diagnose and treat. Previously,
we demonstrated metagenomic sequencing on an Illumina MiSeq replicates the findings of …