Robust Local Thickness Estimation of Sub‐Micrometer Specimen by 4D‐STEM

R Skoupý, DB Boltje, M Slouf, K Mrázová… - Small …, 2023 - Wiley Online Library
A quantitative four‐dimensional scanning transmission electron microscopy (4D‐STEM)
imaging technique (q4STEM) for local thickness estimation across amorphous specimen …

High pressure freezing and cryo-sectioning can be used for protein structure determination by electron diffraction

C Moriscot, G Schoehn, D Housset - Ultramicroscopy, 2023 - Elsevier
Electron diffraction of three-dimensional nanometer sized crystals has emerged since 2013
as an efficient technique to solve the structure of both small organic molecules and model …

[HTML][HTML] CryoEM: a crystals to single particles round-trip

H Hebert - Current opinion in structural biology, 2019 - Elsevier
Highlights•Electron crystallography from 2D-or thin 3D crystals may provide structural
information which is difficult to obtain with other methods.•3D maps are related to the …

Macromolecular nanocrystal structural analysis with electron and X-rays: a comparative review

KP Khakurel, B Angelov, J Andreasson - Molecules, 2019 - mdpi.com
Crystallography has long been the unrivaled method that can provide the atomistic structural
models of macromolecules, using either X-rays or electrons as probes. The methodology …

MicroED sample preparation and data collection for protein crystals

G Bu, BL Nannenga - cryoEM: Methods and Protocols, 2021 - Springer
Abstract Microcrystal Electron Diffraction (MicroED) enables structure determination of very
small crystals that are much too small to be of use for other conventional diffraction …

[PDF][PDF] Scanning electron microscopy as a method for sample visualization in protein X-ray crystallography

EV Beale, AJ Warren, J Trincão, J Beilsten-Edmands… - IUCrJ, 2020 - journals.iucr.org
Developing methods to determine high-resolution structures from micrometre-or even
submicrometre-sized protein crystals has become increasingly important in recent years …

Studying membrane protein structures by MicroED

MW Martynowycz, T Gonen - Structure and Function of Membrane …, 2021 - Springer
Microcrystal electron diffraction (MicroED) enables atomic resolution structures to be
determined from vanishingly small crystals. Soluble proteins typically grow crystals that are …

Low-dose data collection and radiation damage in MicroED

J Hattne - cryoEM: Methods and Protocols, 2021 - Springer
Microcrystal electron diffraction (MicroED) is a technique for structure determination that
relies on the strong interaction of electrons with a minuscule, crystalline sample. While some …

Quantitative electron diffraction and spectroscopy and their application on polymers and 2D materials

C Chen - 2024 - theses.lib.polyu.edu.hk
The determination of structures and phases in functional materials is crucial for
understanding and optimizing their properties. However, traditional direct-imaging electron …

The Study of Cyanobacterial Bicarbonate Transporters and Applications of Microcrystal Electron Diffraction

G Bu - 2021 - search.proquest.com
Cyanobacteria contribute to more than a quarter of the primary carbon fixation worldwide.
They have evolved a CO2 concentrating mechanism (CCM) to enhance photosynthesis …