Global donor and acceptor splicing site kinetics in human cells
RNA splicing is an essential part of eukaryotic gene expression. Although the mechanism of
splicing has been extensively studied in vitro, in vivo kinetics for the two-step splicing …
splicing has been extensively studied in vitro, in vivo kinetics for the two-step splicing …
Caste‐and pesticide‐specific effects of neonicotinoid pesticide exposure on gene expression in bumblebees
Social bees are important insect pollinators of wildflowers and agricultural crops, making
their reported declines a global concern. A major factor implicated in these declines is the …
their reported declines a global concern. A major factor implicated in these declines is the …
[PDF][PDF] The RNA exosome adaptor ZFC3H1 functionally competes with nuclear export activity to retain target transcripts
T Silla, E Karadoulama, D Mąkosa, M Lubas… - Cell reports, 2018 - cell.com
Mammalian genomes are promiscuously transcribed, yielding protein-coding and non-
coding products. Many transcripts are short lived due to their nuclear degradation by the …
coding products. Many transcripts are short lived due to their nuclear degradation by the …
lncRNAs in development and differentiation: from sequence motifs to functional characterization
F Constanty, A Shkumatava - Development, 2021 - journals.biologists.com
The number of long noncoding RNAs (lncRNAs) with characterized developmental and
cellular functions continues to increase, but our understanding of the molecular mechanisms …
cellular functions continues to increase, but our understanding of the molecular mechanisms …
Characterizing RNA stability genome-wide through combined analysis of PRO-seq and RNA-seq data
Background The concentrations of distinct types of RNA in cells result from a dynamic
equilibrium between RNA synthesis and decay. Despite the critical importance of RNA …
equilibrium between RNA synthesis and decay. Despite the critical importance of RNA …
Splicing of long non-coding RNAs primarily depends on polypyrimidine tract and 5′ splice-site sequences due to weak interactions with SR proteins
Z Krchňáková, PK Thakur, M Krausová… - Nucleic acids …, 2019 - academic.oup.com
Many nascent long non-coding RNAs (lncRNAs) undergo the same maturation steps as pre-
mRNAs of protein-coding genes (PCGs), but they are often poorly spliced. To identify the …
mRNAs of protein-coding genes (PCGs), but they are often poorly spliced. To identify the …
Using TTchem-seq for profiling nascent transcription and measuring transcript elongation
LH Gregersen, R Mitter, JQ Svejstrup - Nature protocols, 2020 - nature.com
The dynamics of transcription can be studied genome wide by high-throughput sequencing
of nascent and newly synthesized RNA. 4-thiouridine (4SU) labeling in vivo enables the …
of nascent and newly synthesized RNA. 4-thiouridine (4SU) labeling in vivo enables the …
LNCing RNA to immunity
DC Peltier, A Roberts, P Reddy - Trends in immunology, 2022 - cell.com
Despite an ever-increasing appreciation of how protein-coding genes shape immune
responses, the molecular underpinnings of immune regulation remain incompletely …
responses, the molecular underpinnings of immune regulation remain incompletely …
The multitasking polyA tail: nuclear RNA maturation, degradation and export
A Tudek, M Lloret-Llinares… - … Transactions of the …, 2018 - royalsocietypublishing.org
A polyA (pA) tail is an essential modification added to the 3′ ends of a wide range of RNAs
at different stages of their metabolism. Here, we describe the main sources of …
at different stages of their metabolism. Here, we describe the main sources of …
Genome-wide analysis of lncRNA stability in human
K Shi, T Liu, H Fu, W Li, X Zheng - PLoS computational biology, 2021 - journals.plos.org
Transcript stability is associated with many biological processes, and the factors affecting
mRNA stability have been extensively studied. However, little is known about the features …
mRNA stability have been extensively studied. However, little is known about the features …