Technology in microscopy advances rapidly, enabling increasingly affordable, faster, and
more precise quantitative biomedical imaging, which necessitates correspondingly more …

Achieving complete understanding of any living thing inevitably requires thorough analysis
of both its anatomic and dynamic properties. Live-cell imaging experiments carried out to …

We present TrackMate, an open source Fiji plugin for the automated, semi-automated, and
manual tracking of single-particles. It offers a versatile and modular solution that works out of …

Single-particle tracking (SPT) is often the rate-limiting step in live-cell imaging studies of
subcellular dynamics. Here we present a tracking algorithm that addresses the principal …

Particle tracking is of key importance for quantitative analysis of intracellular dynamic
processes from time-lapse microscopy image data. Because manually detecting and …

Although the highly dynamic and mosaic organization of the plasma membrane is well-
recognized, depicting a resolved, global view of this organization remains challenging. We …

Monitoring the behavior of single molecules in living cells is a powerful approach to
investigate the details of cellular processes. Owing to their optical, chemical and …

In recent years, the deluge of complicated molecular and cellular microscopic images
creates compelling challenges for the image computing community. There has been an …

The past decade has seen an unprecedented data explosion in biology. It has become
evident that in order to take full advantage of the potential wealth of information hidden in the …

Quantitative analysis of biological image data generally involves the detection of many
subresolution spots. Especially in live cell imaging, for which fluorescence microscopy is …