Three-dimensional multiple-particle tracking with nanometric precision over tunable axial ranges

G Sancataldo, L Scipioni, T Ravasenga, L Lanzanò… - Optica, 2017 - opg.optica.org
The precise localization of nanometric objects in three dimensions is essential to identify
functional diffusion mechanisms in complex systems at the cellular or molecular level …

A new detection scheme for multiple object tracking in fluorescence microscopy by joint probabilistic data association filtering

I Smal, W Niessen, E Meijering - 2008 5th IEEE International …, 2008 - ieeexplore.ieee.org
Tracking of multiple objects in biological image data is a challenging problem due largely to
poor imaging conditions and complicated motion scenarios. Existing tracking algorithms for …

Single-molecule tracking and its application in biomolecular binding detection

C Liu, YL Liu, EP Perillo, AK Dunn… - IEEE Journal of …, 2016 - ieeexplore.ieee.org
In the past two decades, significant advances have been made in single-molecule detection
which enables the direct observation of single biomolecules at work in real time and under …

Geometry effect in multi-step crossflow microfluidic devices for red blood cells separation and deformability assessment

V Faustino, D Pinho, SO Catarino, G Minas… - Biomedical …, 2022 - Springer
The efficient separation of blood components using microfluidic systems can help to improve
the detection and diagnosis of several diseases, such as malaria and diabetes. Therefore, a …

High-content screening of primary neurons: ready for prime time

A Daub, P Sharma, S Finkbeiner - Current opinion in neurobiology, 2009 - Elsevier
High-content screening (HCS), historically limited to drug-development companies, is now a
powerful and affordable technology for academic researchers. Through automated routines …

SpatTrack: an imaging toolbox for analysis of vesicle motility and distribution in living cells

FW Lund, MLV Jensen, T Christensen, GK Nielsen… - Traffic, 2014 - Wiley Online Library
The endocytic pathway is a complex network of highly dynamic organelles, which has been
traditionally studied by quantitative fluorescence microscopy. The data generated by this …

Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies

R Lima, MSN Oliveira, T Ishikawa, H Kaji… - …, 2009 - iopscience.iop.org
The current microdevices used for biomedical research are often manufactured using
microelectromechanical systems (MEMS) technology. Although it is possible to fabricate …

Computational processing and analysis of dynamic fluorescence image data

JF Dorn, G Danuser, G Yang - Methods in cell biology, 2008 - Elsevier
With the many modes of live cell fluorescence imaging made possible by the rapid advances
of fluorescent protein technology, researchers begin to face a new challenge: How to …

A new framework for particle detection in low-SNR fluorescence live-cell images and its application for improved particle tracking

L Yang, Z Qiu, AH Greenaway… - IEEE transactions on …, 2012 - ieeexplore.ieee.org
Image denoising and signal enhancement are two common steps to improve particle
contrast for detection in low-signal-to-noise ratio (SNR) fluorescence live-cell images …

Object tracking with particle filtering in fluorescence microscopy images: Application to the motion of neurofilaments in axons

L Yuan, YF Zheng, J Zhu, L Wang… - IEEE Transactions on …, 2011 - ieeexplore.ieee.org
Neurofilaments are long flexible cytoplasmic protein polymers that are transported rapidly
but intermittently along the axonal processes of nerve cells. Current methods for studying …