Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities

A Murray, P Kilbride, MI Gibson - RSC Medicinal Chemistry, 2024 - pubs.rsc.org
Cryopreservation is crucial to fields including immune and stem cell therapies, reproductive
technology, blood banking, regenerative medicine and across all biotechnology. During …

Trehalose modifies the protein profile of ram spermatozoa during cryopreservation

B Jia, S Memon, J Liang, C Lv, Q Hong, G Wu, G Quan - Theriogenology, 2021 - Elsevier
As a magical oligosaccharide, trehalose has been revealed to enhance the post-thaw
quality of stock semen. However, information regarding the cryoprotective mechanism of …

Technologies Used in Production Systems for Santa Inês Sheep: A Systematic Review

AS Bezerra, MAS Santos… - Frontiers in Veterinary …, 2022 - frontiersin.org
This study identifies the number of publications that presented technologies used in the
production systems of Santa Inês sheep in the last 5 years (2017–2021) carried out in Brazil …

Effects of three semen extenders, breeding season month and freezing–thawing cycle on spermatozoa preservation of portuguese Merino sheep

M Fernandes, PR Hernández, J Simões, JP Barbas - Animals, 2021 - mdpi.com
Simple Summary Spermatozoa (SPZ) viability, morphology and the kinetics of fresh and
thawing Portuguese Merino Semen were evaluated at different breeding season months …

Oxidative and nitrosative stress in frozen-thawed pig spermatozoa. II: Effect of the addition of saccharides to freezing medium on sperm function

F Pezo, F Zambrano, P Uribe, J Risopatrón, C Moya… - Cryobiology, 2020 - Elsevier
Saccharides have bioprotective properties, with a high capacity to preserve biological
proteins and membranes during sperm cryopreservation. The aim of this study was to …

Influence of different combinations of permeable and nonpermeable cryoprotectants on the freezing capacity of equine sperm

F Vafaei, H Kohram, A Zareh-Shahne, E Ahmad… - Journal of equine …, 2019 - Elsevier
This study was aimed to evaluate the effect of permeable cryoprotectants in combination
with trehalose or sucrose on the freezing capacity of stallion sperm. For this purpose, the …

[HTML][HTML] Effective cryopreservation of human brain tissue and neural organoids

W Xue, H Li, J Xu, X Yu, L Liu, H Liu, R Zhao… - Cell Reports Methods, 2024 - cell.com
Human brain tissue models and organoids are vital for studying and modeling human
neurological disease. However, the high cost of long-term cultured organoids inhibits their …

Metabolomics analysis of buck semen cryopreserved with trehalose

B Xu, Z Wang, R Wang, G Song, Y Zhang, R Su… - Frontiers in …, 2022 - frontiersin.org
Trehalose is commonly used as an impermeable cryoprotectant for cryopreservation of cells,
but its cryoprotective mechanism has now not but been determined. This study investigated …

Ultrastructural modification of ram sperm frozen with cyclohexanediol and trehalose

J Liang, C Lv, H Yang, Y Zhang, SHA Raza… - Biopreservation and …, 2022 - liebertpub.com
In this study, the effects of trehalose and 1, 3-cyclohexanediol (1, 3-CHD) on the
ultrastructure of frozen-thawed ram sperm were assessed and compared. In the control …

Can amides be alternative cryoprotectors for the preservation of feline semen?

M Madrigal-Valverde, RF Bittencourt… - Cryobiology, 2020 - Elsevier
Sperm cryopreservation is a tool for the conservation of the genetic material of animals of
genetic importance or for species preservation. In the case of domestic cats, this can be …