Third-generation sequencing: the spearhead towards the radical transformation of modern genomics

K Athanasopoulou, MA Boti, PG Adamopoulos… - Life, 2021 - mdpi.com
Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …

New opportunities in plant microbiome engineering for increasing agricultural sustainability under stressful conditions

MS Afridi, MA Javed, S Ali, FHV De Medeiros… - Frontiers in Plant …, 2022 - frontiersin.org
Plant microbiome (or phytomicrobiome) engineering (PME) is an anticipated untapped
alternative strategy that could be exploited for plant growth, health and productivity under …

[HTML][HTML] Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infections

Z Diao, D Han, R Zhang, J Li - Journal of advanced research, 2022 - Elsevier
Background Metagenomic next-generation sequencing (mNGS) has changed the diagnosis
landscape of lower respiratory tract infections (LRIs). With the development of newer …

[HTML][HTML] Plant probiotics–endophytes pivotal to plant health

SS Pandey, R Jain, P Bhardwaj, A Thakur… - Microbiological …, 2022 - Elsevier
Endophytes as a ubiquitous associate of the plant are considered as a promising candidate
for sustainable agriculture. These organisms play a pivotal role in the regulation of the …

[HTML][HTML] Current concepts, advances, and challenges in deciphering the human microbiota with metatranscriptomics

T Ojala, AE Häkkinen, E Kankuri, M Kankainen - Trends in Genetics, 2023 - cell.com
Metatranscriptomics refers to the analysis of the collective microbial transcriptome of a
sample. Its increased utilization for the characterization of human-associated microbial …

Single-cell pathogen diagnostics for combating antibiotic resistance

H Li, K Hsieh, PK Wong, KE Mach, JC Liao… - Nature Reviews …, 2023 - nature.com
Bacterial infections and antimicrobial resistance are a major cause for morbidity and
mortality worldwide. Antimicrobial resistance often arises from antimicrobial misuse, where …

[HTML][HTML] Comparing the significance of the utilization of next generation and third generation sequencing technologies in microbial metagenomics

I Akacin, Ş Ersoy, O Doluca, M Güngörmüşler - Microbiological Research, 2022 - Elsevier
Since the exploration of sequencing began in 2005, third and next-generation sequencing
(TGS and NGS) technologies have fundamentally changed metagenomics research. These …

Agro-industrial waste enzymes: Perspectives in circular economy

LF Rojas, P Zapata, L Ruiz-Tirado - Current Opinion in Green and …, 2022 - Elsevier
According to the Food and Agriculture Organization of the United Nations, approximately 1.3
billion tons of food is wasted each year, equivalent to approximately one-third of world …

[HTML][HTML] Considerations for constructing a protein sequence database for metaproteomics

JA Blakeley-Ruiz, M Kleiner - Computational and structural biotechnology …, 2022 - Elsevier
Mass spectrometry-based metaproteomics has emerged as a prominent technique for
interrogating the functions of specific organisms in microbial communities, in addition to total …

Gut microbiome and metabolome of sea cucumber (Stichopus ocellatus) as putative markers for monitoring the marine sediment pollution in Pahang, Malaysia

SS Wei, CM Yen, IPG Marshall, H Abd Hamid… - Marine Pollution …, 2022 - Elsevier
Antibiotic contamination in the marine environment forms an emerging threat to marine
ecosystems. This study aimed to compare the gut and coelomic microbiota of Stichopus …