After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

Our ability to detect, image, and quantify nanoscopic objects and molecules with visible light
has undergone dramatic improvements over the past few decades. While fluorescence has …

Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

Resolution in optical nanoscopy (or super-resolution microscopy) depends on the
localization uncertainty and density of single fluorescent labels and on the sample's spatial …

Methods based on single-molecule localization and photophysics have brought nanoscale
imaging with visible light into reach. This has enabled single-particle tracking applications …

Video microscopy has a long history of providing insight and breakthroughs for a broad
range of disciplines, from physics to biology. Image analysis to extract quantitative …

Super-resolution localization microscopy methods provide powerful new capabilities for
probing biology at the nanometer scale via fluorescence. These methods rely on two key …

To the Editor: In recent years, there has been a dramatic increase in the use of localization
microscopy: that is, superresolution fluorescence imaging by stochastic photoswitching …

MINFLUX offers a breakthrough in single molecule localization precision, but is limited in
field of view. Here we combine centroid estimation and illumination pattern induced photon …