Harnessing non-Watson–Crick's base pairing to enhance CRISPR effectors cleavage activities and enable gene editing in mammalian cells
Genomic DNA of the cyanophage S-2L virus is composed of 2-aminoadenine (Z), thymine
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …
Application of CRISPR/Cas9 for biomedical discoveries
The C lustered R egions of I nterspersed P alindromic R epeats-Cas9 (CRISPR/Cas9), a
viral defense system found in bacteria and archaea, has emerged as a tour de force genome …
viral defense system found in bacteria and archaea, has emerged as a tour de force genome …
Harnessing noncanonical crRNA for highly efficient genome editing
The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9
system in terms of specificity and multiplexibility. However, its on-target editing efficiency is …
system in terms of specificity and multiplexibility. However, its on-target editing efficiency is …
The eukaryotic way to defend and edit genomes by sRNA‐targeted DNA deletion
EC Swart, M Nowacki - Annals of the New York Academy of …, 2015 - Wiley Online Library
While there is currently burgeoning interest in the application of the CRISPR/Cas (clustered
regularly interspaced short palindromic repeats/CRISPR‐associated genes) to genome …
regularly interspaced short palindromic repeats/CRISPR‐associated genes) to genome …
Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing
technology to functional genetics and human therapeutic applications. Here, we show that …
technology to functional genetics and human therapeutic applications. Here, we show that …
[HTML][HTML] Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity
EM Anderson, A Haupt, JA Schiel, E Chou… - Journal of …, 2015 - Elsevier
The discovery that the bacterial clustered regularly interspaced short palindromic repeats
(CRISPR)–CRISPR-associated protein 9 (Cas9) acquired immune system can be utilized to …
(CRISPR)–CRISPR-associated protein 9 (Cas9) acquired immune system can be utilized to …
An engineered xCas12i with high activity, high specificity, and broad PAM range
H Zhang, X Kong, M Xue, J Hu, Z Wang, Y Wei… - Protein & …, 2023 - academic.oup.com
The clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems,
including type II Cas9 and type V Cas12 systems, which serve in the adaptive immunity of …
including type II Cas9 and type V Cas12 systems, which serve in the adaptive immunity of …
CRISPR-based technologies for the manipulation of eukaryotic genomes
The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …
advances in the life sciences that have grown from the ability to edit genomes within living …
CRISPR-Cas12a has widespread off-target and dsDNA-nicking effects
Cas12a (Cpf1) is an RNA-guided endonuclease in the bacterial type VA CRISPR-Cas anti-
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …
phage immune system that can be repurposed for genome editing. Cas12a can bind and cut …
A more efficient CRISPR-Cas12a variant derived from Lachnospiraceae bacterium MA2020
MH Tran, H Park, CL Nobles, P Karunadharma… - … Therapy-Nucleic Acids, 2021 - cell.com
CRISPR effector proteins introduce double-stranded breaks into the mammalian genome,
facilitating gene editing by non-homologous end-joining or homology-directed repair. Unlike …
facilitating gene editing by non-homologous end-joining or homology-directed repair. Unlike …