We have expressed dhfr alleles of Plasmodium falciparum in the budding yeast,
Saccharomyces cerevisiae, and used this yeast model to identify single amino acid …

In Plasmodium falciparum, resistance to folate inhibitors like pyrimethamine is mediated by
point mutations in the target gene dihydrofolate reductase (dhfr). The resistance to …

Abstract Background Recently, Plasmodium falciparum parasites bearing Pfdhfr I164L
single mutation were found in Madagascar. These new mutants may challenge the use of …

Inhibitors of dihydrofolate reductase (DHFR) have been a mainstay of chemotherapy of
falciparum malaria for> 50 years. Unfortunately, point mutations in DHFR are the major …

Plasmodium falciparum, the protozoan that causes the most lethal form of human malaria,
has been controlled principally by two safe, affordable drugs, chloroquine and sulfadoxine …

Two new dihydrofolate reductase (DHFR) mutations were recently discovered in
Plasmodium falciparum samples from an area of Bolivia with high rates of in vivo resistance …

Pyrimethamine and cycloguanil are competitive inhibitors of the Plasmodium enzyme
dihydrofolate reductase (DHFR). They have been effective treatments for malaria, but rapid …

Differential resistance of Plasmodium falciparum to the antimalarial drugs pyrimethamine
and cycloguanil (the active metabolite of proguanil) results from specific mutations in …

The nucleotide sequence of the dihydrofolate reductase-thymidylate synthase (DHFR-TS)
gene in pyrimethamine-resistant (Pyr R) mutants of Plasmodium falciparum selected in vitro …

To overcome the declining efficacy of the 4-aminoquinolines in Papua New Guinea,
sulfadoxine/pyrimethamine (SP) was combined with the 4-aminoquinolines as the first line …