Inhibitors of dihydrofolate reductase (DHFR) have been a mainstay of chemotherapy of
falciparum malaria for> 50 years. Unfortunately, point mutations in DHFR are the major …

Pyrimethamine and cycloguanil are competitive inhibitors of the Plasmodium enzyme
dihydrofolate reductase (DHFR). They have been effective treatments for malaria, but rapid …

Two new dihydrofolate reductase (DHFR) mutations were recently discovered in
Plasmodium falciparum samples from an area of Bolivia with high rates of in vivo resistance …

Single and multiple mutations at residues 16, 51, 59, 108, and 164 of Plasmodium
falciparum dihydrofolate reductase (pfDHFR) have been linked to antifolate resistance in …

Plasmodium falciparum strains bearing quadruple mutations of dihydrofolate reductase-
thymidylate synthase (PfDHFR-TS) at codons 51, 59, 108, and 164 are highly resistant to …

We describe the system for screening the effective antifolate antimalarials that uses the
recombinant Plasmodium falciparum DHFR domain of the bifunctional DHFR-TS expressed …

Resistance to antifolates of the malaria parasite Plasmodium falciparum stems from
stepwise mutations of the target enzyme dihydrofolate reductase (DHFR). New drugs can be …

A simple and effective system has been developed from which a number of Plasmodium
falciparum dihydrofolate reductase (pfDHFR) mutants conferring resistance to antifolates …

The codon for serine residue 108 of thePlasmodium falciparumdihydrofolate reductase gene
was replaced with those for the other 19 amino acids. Except for the Lys108mutant, which …

In Plasmodium falciparum, resistance to folate inhibitors like pyrimethamine is mediated by
point mutations in the target gene dihydrofolate reductase (dhfr). The resistance to …