NK Roy, RK Ghosh, J Das - Microbiology, 1982 - microbiologyresearch.org
The synthesis of alkaline phosphatase by two strains of Vibrio cholerae belonging to the Inaba and Ogawa serotypes has been examined in relation to the phosphate concentration …
Materials and Methods Bacterial Strains.—A strain of E. coli K12, designated C4Fi, was obtained from Dr. AM Torriani of the Massa-chusetts Institute of Technology. This strain …
Alkaline phosphatases (AP) are widely distributed in nature, and generally have a dimeric structure. However, there are indications that either monomeric or multimeric bacterial forms …
R Olsen - Analytical Biochemistry, 1970 - Elsevier
Escherichia coZi alkaline phosphatase is a heat-stable, mukimeCc:, metallo-enzyme localized to the periplasmic space of the bacterial cell (I-5). The protein's stability and its …
MJ Schlesinger, L Andersen - … of the New York Academy of …, 1968 - Wiley Online Library
Highly purified preparations of alkaline phosphatase from Escherichia coli are found to contain several distinct enzymatically active forms when analyzed by starch-gel …
LB Pett, AM Wynne - Biochemical Journal, 1938 - ncbi.nlm.nih.gov
Materials and methods Conditions were the same as thosedescribed by Pett & Wynne [1933] with the following variations. The reaction volume was only 5 ml. instead of 10, and …
PM Kelley, PA Neumann, K Shriefer, F Cancedda… - Biochemistry, 1973 - ACS Publications
Materials and Methods Purification of Isozymes. E. coli alkaline phosphatase isozyme 3 (see Figure 1 for nomenclature) was purified from cells (strain ATCC 27257) grown in a 100-1 …
Robert T. Simpson, f Bert L. Vallee, and George H. Tait {abstract: A rapid, single-step purification of the alka-line phosphatase of Escherichia coli using salt gradient …
LA Heppel, DR Harkness, RJ Hilmoe - Journal of Biological Chemistry, 1962 - Elsevier
Materials and Methods A number of samples of alkaline phosphatase were examined. The preparations are designated as follows: GL, from Drs. Garen and Levinthal (5)(1230 units …