[HTML][HTML] Development of a Single-Chain Fragment Variable that Binds to the SARS-CoV-2 Spike Protein Produced by Genetically Modified Lactic Acid Bacteria

S Oshima, F Namai, T Sato, T Shimosato - Molecular Biotechnology, 2024 - Springer
SARS-CoV-2 enters cells via binding of the surface-exposed spike protein RBD to host cell
ACE2 receptors. Therefore, in this study, we designed a scFv (single-chain fragment …

[HTML][HTML] A vaccine based on the receptor-binding domain of the spike protein expressed in glycoengineered Pichia pastoris targeting SARS-CoV-2 stimulates …

B Liu, Y Yin, Y Liu, T Wang, P Sun, Y Ou, X Gong… - Engineering, 2022 - Elsevier
In 2020 and 2021, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel
coronavirus, caused a global pandemic. Vaccines are expected to reduce the pressure of …

[HTML][HTML] Isolation of SARS-CoV-2-blocking recombinant antibody fragments and characterisation of their binding to variant spike proteins

D Antoine, M Mohammadi, CE McDermott… - Frontiers in …, 2022 - frontiersin.org
COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2. From its initial
appearance in Wuhan, China in 2019, it developed rapidly into a global pandemic. In …

Comparison of expression of recombinant binding region (RBD) of SARS-CoV-2 virus in Escherichia coli and insect cells, evaluation of recombinant protein function …

Z Rahbar, S Nazarian, R Dorostkar… - New Cellular and …, 2023 - ncmbjpiau.ir
Abstract Aim and Background: COVID-19, an infectious viral disease caused by severe
acute respiratory syndrome (SARS-CoV-2) with more than 260 million infections as of …

Generation and utility of a single-chain fragment variable monoclonal antibody platform against a baculovirus expressed recombinant receptor binding domain of …

R Salem, AA El-Kholy, FR Waly, D Ayman, A Sakr… - Molecular …, 2022 - Elsevier
As the second wave of COVID-19 launched, various variants of Severe Acute Respiratory
Syndrome Coronavirus 2 (SARS-CoV-2) have emerged with a dramatic global spread …

Construction and immunogenic studies of a mFc fusion receptor binding domain (RBD) of spike protein as a subunit vaccine against SARS-CoV-2 infection

X Qi, B Ke, Q Feng, D Yang, Q Lian, Z Li, L Lu… - Chemical …, 2020 - pubs.rsc.org
Herein, we report that a recombinant fusion protein, containing a 457 amino acid SARS-CoV-
2 receptor binding domain (RBD, residues 319–541) and a mouse IgG1 Fc domain, could …

[HTML][HTML] Purification and characterization of the receptor‐binding domain of SARS‐CoV‐2 spike protein from Escherichia coli

Y He, J Qi, L Xiao, L Shen, W Yu… - Engineering in Life …, 2021 - Wiley Online Library
SARS‐CoV‐2 is responsible for a disruptive worldwide viral pandemic, and renders a
severe respiratory disease known as COVID‐19. Spike protein of SARS‐CoV‐2 mediates …

Expression, purification, and study on the efficiency of a new potent recombinant scFv antibody against the SARS-CoV-2 spike RBD in E. coli BL21

F Yaghoobizadeh, MR Ardakani, MM Ranjbar… - Protein Expression and …, 2023 - Elsevier
Many efforts have been made around the world to combat SARS-CoV-2. Among these are
recombinant antibodies considered to be suitable as an alternative for some diagnostics …

Recombinant RBD of the SARS-CoV-2 Spike Protein: Production in Escherichia coli Cells, Binding to Antibodies, and Antiviral Activity

MS Gromova, AV Gromov, TM Grunina… - Molecular Genetics …, 2023 - Springer
The aim of the study is to synthesize a recombinant protein in Escherichia coli cells that
carries the receptor-binding domain (RBD) of Spike protein of SARS CoV-2, with antiviral …

Bacterial expression and purification of functional recombinant SARS-CoV-2 spike receptor binding domain

J Prahlad, LR Struble, WE Lutz, SA Wallin, S Khurana… - BioRxiv, 2021 - biorxiv.org
The COVID-19 pandemic caused by SARS-CoV-2 has applied significant pressure on
overtaxed healthcare around the world, underscoring the urgent need for rapid diagnosis …