Multimodal single-cell assays provide high-resolution snapshots of complex cell
populations, but are mostly limited to transcriptome plus an additional modality. Here, we …

Single-cell CRISPR screens enable the exploration of mammalian gene function and
genetic regulatory networks. However, use of this technology has been limited by reliance …

Pooled CRISPR screens coupled with single-cell RNA-sequencing have enabled systematic
interrogation of gene function and regulatory networks. Here, we introduce Cas13 RNA …

CRISPR-based genetic screens are accelerating biological discovery, but current methods
have inherent limitations. Widely used pooled screens are restricted to simple readouts …

Assigning functions to genes and learning how to control their expression are part of the
foundation of cell biology and therapeutic development. An efficient and unbiased method to …

We present a combinatorial indexing method, PerturbSci-Kinetics, for capturing whole
transcriptomes, nascent transcriptomes and single guide RNA (sgRNA) identities across …

CRISPR pools are being widely employed to identify gene functions. However, current
technology, which utilizes DNA as barcodes, permits limited phenotyping and bulk-cell …

We report a robust, versatile approach called CRISPR live-cell fluorescent in situ
hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad …

Clustered regularly interspaced short palindromic repeats (CRISPR) screening is a powerful
toolset for investigating diverse biological processes. Most CRISPR screens to date have …

Identification of novel functional domains and characterization of detailed regulatory
mechanisms in cancer-driving genes is critical for advanced cancer therapy. To date …