Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system
Labeling and tracing of specific sequences in living cells has been a major challenge in
studying the spatiotemporal dynamics of native chromatin. Here we repurposed the …
studying the spatiotemporal dynamics of native chromatin. Here we repurposed the …
[HTML][HTML] High-throughput single-cell sequencing with linear amplification
Conventional methods for single-cell genome sequencing are limited with respect to
uniformity and throughput. Here, we describe sci-L3, a single-cell sequencing method that …
uniformity and throughput. Here, we describe sci-L3, a single-cell sequencing method that …
Live cell imaging of low-and non-repetitive chromosome loci using CRISPR-Cas9
Imaging chromatin dynamics is crucial to understand genome organization and its role in
transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been …
transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been …
MIC-Drop: A platform for large-scale in vivo CRISPR screens
CRISPR-Cas9 can be scaled up for large-scale screens in cultured cells, but CRISPR
screens in animals have been challenging because generating, validating, and keeping …
screens in animals have been challenging because generating, validating, and keeping …
Single-cell lineage tracing by integrating CRISPR-Cas9 mutations with transcriptomic data
Recent studies combine two novel technologies, single-cell RNA-sequencing and CRISPR-
Cas9 barcode editing for elucidating developmental lineages at the whole organism level …
Cas9 barcode editing for elucidating developmental lineages at the whole organism level …
Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters
C Umkehrer, F Holstein, L Formenti, J Jude… - Nature …, 2021 - nature.com
We developed a functional lineage tracing tool termed CaTCH (CRISPRa tracing of clones
in heterogeneous cell populations). CaTCH combines precise clonal tracing of millions of …
in heterogeneous cell populations). CaTCH combines precise clonal tracing of millions of …
Functional genomics via CRISPR–Cas
RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated
Cas proteins have recently emerged as versatile tools to investigate and engineer the …
Cas proteins have recently emerged as versatile tools to investigate and engineer the …
Single-cell RNA counting at allele and isoform resolution using Smart-seq3
Large-scale sequencing of RNA from individual cells can reveal patterns of gene, isoform
and allelic expression across cell types and states. However, current short-read single-cell …
and allelic expression across cell types and states. However, current short-read single-cell …
Optimal marker gene selection for cell type discrimination in single cell analyses
Single-cell technologies characterize complex cell populations across multiple data
modalities at unprecedented scale and resolution. Multi-omic data for single cell gene …
modalities at unprecedented scale and resolution. Multi-omic data for single cell gene …
CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
Current methods can illuminate the genome-wide activity of CRISPR–Cas9 nucleases, but
are not easily scalable to the throughput needed to fully understand the principles that …
are not easily scalable to the throughput needed to fully understand the principles that …