It has been just over 10 years since the initial description of transposase-based methods to
prepare high-throughput sequencing libraries, or “tagmentation,” in which a hyperactive …

The continual drop in sequencing costs and the steady improvement of platforms and
protocols have allowed single-cell sequencing to become widely used over the past few …

Single-cell sequencing provides information that is not confounded by genotypic or
phenotypic heterogeneity of bulk samples. Sequencing of one molecular type (RNA …

Single-cell sequencing is widely used in biological and medical studies. However, its
application with multiple samples is hindered by inefficient sample processing, high …

A revolution in cellular measurement technology is under way: For the first time, we have the
ability to monitor global gene regulation in thousands of individual cells in a single …

Abstract Cleavage Under Targets and Tagmentation (CUT&Tag) is an antibody-directed
transposase tethering strategy for in situ chromatin profiling in small samples and single …

Most genome-wide assays provide averages across large numbers of cells, but recent
technological advances promise to overcome this limitation. Pioneering single-cell assays …

In the last decade, molecular and cellular technologies have evolved to give rise to the era
of single-cell genomics, which allows the simultaneous measurement of thousands of genes …

Identification of cell populations often relies on manual annotation of cell clusters using
established marker genes. However, the selection of marker genes is a time-consuming …

Background A fundamental characteristic of multicellular organisms is the specialization of
functional cell types through the process of differentiation. These specialized cell types not …