In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …

The number of reports per year on single-molecule imaging experiments has grown roughly
exponentially since the first successful efforts to optically detect a single molecule were …

Precise imaging of cellular and subcellular structures and dynamic processes in live cells is
crucial for fundamental research in life sciences and in medical applications. Non …

Quantitative single-cell analysis enables the characterization of cellular systems with a level
of detail that cannot be achieved with ensemble measurement. In this Feature we explore …

Quantitative optical microscopy—an emerging, transformative approach to single-cell
biology—has seen dramatic methodological advancements over the past few years …

Single cell analysis is an emerging field requiring a high level interdisciplinary collaboration
to provide detailed insights into the complex organisation, function and heterogeneity of life …

The transition of single-molecule fluorescence detection and imaging from in vitro to living
cells has greatly enriched our knowledge on the behavior of single biomolecules in their …

The optical microscope launched the entire discipline of cell biology some 300 years ago,
with Antony van Leeuwenhoek's observations of plant and animal cells and single-celled …

Since the word microscopy was coined in 1656, the evolution of the instrument has had a
long and convoluted history. Plagued with problems of chromatic aberration, spherical …

Conspectus Innovations in light microscopy have tremendously revolutionized the way
researchers study biological systems with subcellular resolution. In particular, fluorescence …