The gold standard for clinical diagnosis of bacterial lower respiratory infections (LRIs) is
culture, which has poor sensitivity and is too slow to guide early, targeted antimicrobial …

Background Metagenomic sequencing of respiratory microbial communities for pathogen
identification in pneumonia may help overcome the limitations of culture-based methods. We …

Urinary tract infections (UTIs) are among the most common acquired bacterial infections in
humans. The current gold standard method for identification of uropathogens in clinical …

Objectives The introduction of metagenomic sequencing to diagnostic microbiology has
been hampered by slowness, cost and complexity. We explored whether MinION nanopore …

Next-generation whole-genome sequencing is essential for high-resolution surveillance of
bacterial pathogens, for example, during outbreak investigations or for source tracking and …

Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …

Fast and accurate identification of microbial pathogens is critical for the proper treatment of
infections. Traditional culture‐based diagnosis in clinics is increasingly supplemented by …

We developed a metagenomic next-generation sequencing (mNGS) test using cell-free DNA
from body fluids to identify pathogens. The performance of mNGS testing of 182 body fluids …

Objectives Genome sequencing will be increasingly used in the clinical setting to tailor
antimicrobial prescribing and inform infection control outbreaks. A recent technological …

Background Direct and indirect selection pressures imposed by antibiotics and co-selective
agents and horizontal gene transfer are fundamental drivers of the evolution and spread of …