Transcription is a highly dynamic process. Consequently, we have developed native
elongating transcript sequencing technology for mammalian chromatin (mNET-seq), which …

The transcription cycle of RNA polymerase II (Pol II) correlates with changes to the
phosphorylation state of its large subunit C-terminal domain (CTD). We recently developed …

Major features of transcription by human RNA polymerase II (Pol II) remain poorly defined
due to a lack of quantitative approaches for visualizing Pol II progress at nucleotide …

Many features of how gene transcription occurs in human cells remain unclear, mainly
because of a lack of quantitative approaches to follow genome transcription with nucleotide …

Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …

Mammalian chromatin is the site of both RNA polymerase II (Pol II) transcription and coupled
RNA processing. However, molecular details of such co-transcriptional mechanisms remain …

The advent of next-generation sequencing, and in particular RNA-sequencing (RNA-seq),
technologies has expanded our knowledge of the transcriptional capacity of human and …

Recent studies of transcription have revealed a level of complexity not previously
appreciated even a few years ago, both in the intricate use of post-initiation control and the …

Transcription occurring at gene loci results in accumulation of mature RNA molecules (ie,
mRNAs) that can be easily assayed by RT-PCR or RNA sequencing. However, the steady …

Background Early application of second-generation sequencing technologies to transcript
quantitation (RNA-seq) has hinted at a vast mammalian transcriptome, including transcripts …